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Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity

2021Artigo científico Acesso aberto
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dc.contributor.authorIvanov, Marija
dc.contributor.authorKannan, Abhilash
dc.contributor.authorStojković, Dejan
dc.contributor.authorGlamočlija, Jasmina
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSanglard, Dominique
dc.contributor.authorSoković, Marina
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-11-22T14:21:48Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-11-22T14:21:48Z
dc.date.issued2021
dc.description.abstractCandida albicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125–0.35 mg/mL while eucalyptol was active in the range of 2–23 mg/mL. The results showed camphor’s potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.en_EN
dc.description.sponsorshipThis research is funded by the Serbian Ministry of Education, Science and Technological Development [Contract No. 451-03-68/2020-14/200007]. The authors are grateful to the FEMS for providing FEMS Research and Training Grant [FEMS-GO-2017-015] to Mrs Marija Ivanov for her visit to the Institute of Microbiology, University Hospital Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, Switzerland. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO [UIDB/00690/2020] and to the national funding by FCT, P.I., through the institutional scientific employment program-contract for R. Calhelha’s contract.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationIvanov, Marija; Kannan, Abhilash; Stojković, Dejan S.; Glamočlija, Jasmina; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Sanglard, Dominique; Soković, Marina (2021). Camphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. International Journal of Molecular Sciences. ISSN 1661-6596. 22:2, p. 1-14en_EN
dc.identifier.doi10.3390/ijms22020483en_EN
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10198/24252
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationMountain Research Center
dc.subjectAntifungal activityen_EN
dc.subjectCamphoren_EN
dc.subjectCytotoxicityen_EN
dc.subjectEfflux pumpsen_EN
dc.subjectEucalyptolen_EN
dc.subjectTerpenoidsen_EN
dc.subjectVirulence factorsen_EN
dc.titleCamphor and eucalyptol—anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicityen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCalhelha
person.familyNameFerreira
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication2d5d1a41-7561-4a01-871c-b4c97da35053
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

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