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Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol

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dc.contributor.authorMoreno, Abília
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2015-11-24T11:13:56Z
dc.date.available2015-11-24T11:13:56Z
dc.date.issued2015
dc.description.abstractMushrooms are well known for their richness in bioactive molecules such as antioxidants. Phenolic compounds, in particular phenolic acids, have been the most widely studied molecules regarding these effects [1]. Nevertheless, other molecules present in mushrooms, such as ergosterol, can also display bioactive properties [2]. Although being this high-value molecule more associated with hypocholesterolemic, antimicrobial and anti-inflammatory effects [2], it is also relevant to screen other bioactivities, such as antioxidant activity, either of the pure molecule or of mycosterols' rich extracts containing it. Agaricus bisporus L. is the most consumed mushroom worldwide, being ergosterol the most abundant mycosterol in its sterol fraction (represents almost 90%) [3]. Herein, A. bisporus ethanol and hexane extracts were prepared by Soxhlet and ultrasound-assisted extraction (amplitude: 75%;sonication time: 15 min), and further evaluated for their antioxidant activity. The in vitro assays used were: i) 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH) assay, to evaluate the scavenging activity; and ii) ferricyanide Prussian blue assay, to evaluate the reducing power. The obtained extracts were characterized in terms of ergosterol content by HPLC-UV. The antioxidant activity of pure ergosterol was also assessed. The extracts obtained by Soxhlet showed higher antioxidant activity than the ones obtained by ultrassonication, which is in agreement with the higher leveis of ergosterol found in the flrst extracts (677 and 186 mg/100 g dw for ethanol and hexane extracts, respectively). Ethanol extracts revealed higher antioxidant activity than the hexane exfracts, which is also in agreement with the higher ergosterol content found in both samples (677 and 672 mg/100 g dw for Soxhlet and ultrassonication extracts, respectively). The pure ergosterol also showed antioxidant activity (e. g., DPPH ECso value = 0.46 mg/mL). Overall, the ethanol extract obtained by Soxhlet gave the highest DPPH scavenging activity (EC5o = 2.2 mg/mL) and reducing power (ECsa = 0.8 mg/mL), while the hexane extract obtained by ultrassonication revealed the lowest DPPH scavenging activity (ECso = 16.7 mg/mL) and reducing power (EC5o= 2.2 mg/mL).pt_PT
dc.identifier.citationMoreno, Abília; Heleno, Sandrina A.; Barros, Lillian; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2015). Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol. In 2nd Symposium on Medicinal Chemistry of University of Minhopt_PT
dc.identifier.urihttp://hdl.handle.net/10198/12380
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNORTE-07-0124-FEDER-000014pt_PT
dc.relationNORTE-07-0162-FEDER-000050pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleAntioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterolpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F50020%2F2013/PT
oaire.citation.title2nd Symposium on Medicinal Chemistry of University of Minhopt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameHeleno
person.familyNameBarros
person.familyNameBarreiro
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id3418-47D5-5746
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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