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Evaluation of minimum inhibition concentrations of plant against environmental fungi and dermatophytes

2021Conference object Open access
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dc.contributor.authorAfonso, Tiago B.
dc.contributor.authorMelo, Adma N.F.
dc.contributor.authorCarvalho, Marta
dc.contributor.authorRibeiro, Tânia
dc.contributor.authorPinto, Miguel M.
dc.contributor.authorBarros, Lillian
dc.contributor.authorCarocho, Márcio
dc.contributor.authorTavaria, Freni K.
dc.contributor.authorTeixeira, Paula
dc.contributor.authorPintado, Manuela
dc.date.accessioned2022-02-22T10:56:53Z
dc.date.available2022-02-22T10:56:53Z
dc.date.issued2021
dc.description.abstractThe outbreak of COVID-19 disease eaused by SARS-CoV-2 has led the seientifie eompmnity to seareh for new alternatives to help eontrol the virus. In this context, the use of face masks has been reeommended as a preventive measure against the spread of SARS-CoV-2. The application of antimierobial agents in the tissues used to produee masks would be an additional hurdle on the prevention of other respiratory infeetions and seeondary fungaI infeetions that oeeur from touehing eontaminated masks. In this eontext, impregnation of the fabries with plant derived extraets is an attraetive approaeh sinee they are potential1y safe, free of adverse side effeets, and powerful antimierobials. The objeetive of this work was to study fifteen plant extraets to seleet the most effieient against environmental fungi and some dermatophytes. MaterialslMethods: The minimum inhibitory eoneentration (MIC) of eaeh plant derived extraet was determined by broth mierodilution in 96-wells mierotiter plates. Coneentrations ranging from 50 mg/mL to 1.5625 mg/mL were tested against lhe different fungi. Briefly, 100 mL of a 105 spores/mL solution were added to 100 mL of eaeh extraet eoneentration. Afier 48 h, 10 mL of eaeh well were plated onto Sabouraud Dextrose Agar. Cultivation was earried out at 25 °C for 3 days for the environmental fungi and 7 days for the dermatophytes. The MICs were regarded as the lowest coneentrations that did not allow any visible growth when eompared with the eontrol sets. Results: The results for the fifteen plant extraets tested against nine environmental fungai species revealed that only one extraet was able to inbibit fungai growth of two of (hese fungi (Cladosporium sp. and F. verticillioides) at a eoneentration of 50 mg/mL. The results against the two filamentous fungi dermatophytes (T. mentagrophytes and M canis) revealed these fungi to be the lllf'st susceptible to the extraets tested. Most of the extraels inhibited dermatophyte growth at eoneettrations < 50 mg/mL with the lowest MIC being registered at 3.125 mg/mL. Results for the yeast M. furfur revealed that only three plant derived extraets inhibited its growth with eoncentrations between 30 and 50 mg/mL. Conclusion: The plant extraets tested against environmental fungi and dermatophytes revealed to be more efficient against filamentous fungi dermatophyte growth. Environmental fungi followed by lhe dermatophyte yeast, showed the highest resislanee to these pIant extracts.pt_PT
dc.description.sponsorshipProject financed by the European Fund for Regional Development (Fundo Europeu de Desenvolvimento Regional (FEDER)) through the Programa Operacional Regional Norte 2020, within the "PlantCovid" project, NORTE-01-02B7-FEDER-054870pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAfonso, Tiago B.; Melo, Adma N.F.; Carvalho, Marta; Ribeiro, Tânia; Pinto, Miguel M.; Barros, Lillian; Carocho, Márcio; Tavaria, Freni; Teixeira, Paula; Pintado, Manuela (2021). Evaluation of minimum inhibition concentrations of plant against environmental fungi and dermatophytes. In 4th International Congress of Bíochemistry and Microbiology Applied Technologie: book of abstracts. Hammametpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/25102
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAEAE Congresspt_PT
dc.relationNORTE-01-02B7-FEDER-054870pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEnvironmental fungipt_PT
dc.subjectDermatophytespt_PT
dc.titleEvaluation of minimum inhibition concentrations of plant against environmental fungi and dermatophytespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceHammametpt_PT
oaire.citation.endPage179pt_PT
oaire.citation.startPage179pt_PT
oaire.citation.title4th International Congress of Bíochemistry and Microbiology Applied Technologie: book of abstractspt_PT
person.familyNameBarros
person.familyNameCarocho
person.givenNameLillian
person.givenNameMárcio
person.identifier469085
person.identifierawbWiiMAAAAJ&hl
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id5614-005B-5CF1
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-8978-4547
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id52463229800
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublication46b39e1f-2674-4be1-a96a-c2e5792e6979
relation.isAuthorOfPublication.latestForDiscovery3af07ffe-f914-48ba-a5d5-efcf70fdce01

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