Cynara Scolymus clustering in plant food supplements by high resolution melting analysis

Batista, Andreia; Costa, Joana; Fernandes, Telmo J.R.; Amaral, Joana S.; Ramos, Fernando; Oliveira, Beatriz; Mafra, Isabel

Publication

Cynara Scolymus clustering in plant food supplements by high resolution melting analysis

2015Conference object

dc.contributor.author	Batista, Andreia
dc.contributor.author	Costa, Joana
dc.contributor.author	Fernandes, Telmo J.R.
dc.contributor.author	Amaral, Joana S.
dc.contributor.author	Ramos, Fernando
dc.contributor.author	Oliveira, Beatriz
dc.contributor.author	Mafra, Isabel
dc.date.accessioned	2018-04-09T11:12:28Z
dc.date.available	2018-04-09T11:12:28Z
dc.date.issued	2015
dc.description.abstract	Plant food supplements (PFS) have been regarded with special interest since they are concentrated sources of nutrients or other substances with a nutritional or physiological effect, contributing to a better homeostatic balance. Weight-loss PFS, one of the most consumed PFS, may include Cynara scolymus (artichoke) as an ingredient due to its antioxidant, diuretic, choleretic and hepatoprotective properties [1]. Considered as foods under the EU Directive 2002/46/EC, the PFS are not usually subjected to any safety assessment prior to their commercialisation. This can lead to adulteration issues, such as accidental swap of plants or deliberate substitution of higher cost botanicals by other similar, but cheaper species. Thus, to ensure consumer’s safety, the development of analytical methods for the correct identification of different plant species in PFS has become essential. Until now, DNA-based methods have been reported as highly appropriate tools for plant authentication [2]. The aim of this study was to discriminate C. scolymus from other Cynara spp. using real-time polymerase chain reaction (PCR) coupled to high resolution melting (HRM) analysis. For this purpose, different Cynara species (C. scolymus, C. syriaca, C. cardunculus and C. humilis) were obtained from Portuguese and French Germplasm Banks. A total of 8 PFS tablets for weight-loss containing artichoke were acquired at local herbal stores. DNA from plant material and PFS was extracted using the commercial NucleoSpin Plant II kit. Former to DNA extraction, PFS were pre-treated with a phosphate buffer 1M (pH8, 15% ethanol) to enhance the purity and quality of the extracts. The specificity and sensitivity of the designed primers targeting C. scolymus were assayed by qualitative PCR and real-time PCR with HRM analysis. The application of the specific PCR assay was successful in the detection of Cynara spp. in some of the PFS samples. The results of real-time PCR with HRM analysis showed that different Cynara spp. were included in three distinct clusters with a level of confidence above 99.4%, thus discriminating artichoke from other Cynara species. The proposed HRM analysis allowed confirming the unequivocal presence of C. scolymus in the tested PFS with high level of confidence (>98.8%). To our knowledge, this is the first successful attempt for the rapid discrimination of C. scolymus in PFS.	pt_PT
dc.description.sponsorship	This work was supported by European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia) through project EXPL/DTPSAP/1438/2013 and PEst-C/EQB/LA0006/2013	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Batista, A.; Costa, Joana; Fernandes, T.J.R. Amaral, J.S., Ramos, F.; Oliveira, M.B.P.P.; Mafra, I. (2015). Cynara Scolymus clustering in plant food supplements by high resolution melting analysis. In IJUP 2015 8th Meeting of Young Researchers of University of Porto: Book of abstracts. Porto	pt_PT
dc.identifier.uri	http://hdl.handle.net/10198/16776
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.title	Cynara Scolymus clustering in plant food supplements by high resolution melting analysis	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FDTP-SAP%2F1438%2F2013/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FEQB%2FLA0006%2F2013/PT
oaire.citation.conferencePlace	Porto, Portugal	pt_PT
oaire.citation.endPage	144	pt_PT
oaire.citation.startPage	144	pt_PT
oaire.citation.title	IJUP 2015 Book of abstracts of the 8th Meeting of Young Researchers of University of Porto	pt_PT
oaire.fundingStream	COMPETE
oaire.fundingStream	COMPETE
person.familyName	Amaral
person.givenName	Joana S.
person.identifier.ciencia-id	5319-7DE8-BEDA
person.identifier.orcid	0000-0002-3648-7303
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT
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