Isolation and characterization by asymmetric PCR of the ENDO1G gene for Glucan endo-1,3-b-D-glucosidase in Phytophthora cinnamomi associated whit the ink disease of Castanea sativa Mill.

Meirinho, Sofia G.; Carvalho, Marisa; Dominguez, Ángel; Choupina, Altino

http://hdl.handle.net/10198/5249

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Abstract(s)

Ink disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-b-D-glucosidase gene obtained by amplification, using conserved primers and the full-length gene sequence by flanking this known sequence by asymmetric PCR. This fragment was obtained from genomic DNA of Phytophthora cinnamomi isolated in the European Regions of Castilla-Leon (Spain) and Trás-os-Montes (Portugal) and associated with the ink disease of Castanea sativa Mill.

Keywords

Phytophthora cinnamomi Glucan endo-1,3-b-D-glucosidase Asymmetric PCR

URI

http://hdl.handle.net/10198/5249

Citation

Meirinho, Sofia; Carvalho, Marisa; Dominguez, Angel; Choupina, Altino (2010). Isolation and characterization by asymmetric PCR of the ENDO1G gene for Glucan endo-1,3-b-D-glucosidase in Phytophthora cinnamomi associated whit the ink disease of Castanea sativa Mill. Brazilian Archives of Biology and Technology. ISSN 1516-8913. 53:3, p. 513-518