Orientador(es)
Resumo(s)
Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to
prevent pathogen infections. The present study aimed to analyze the e ect of chitosan solution and
chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic
composition, antioxidant potential and antibacterial activity of its individual grape components.
Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles,
and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated
with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of
the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility
method was performed using the Kirby-Bauer disc di usion method against multidrug-resistant
bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant
and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed
the highest antioxidant and antimicrobial activities. The studied individual components obtained
from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and
pharmaceutical industries as natural food preservers and antibiotic adjuvants.
Descrição
Palavras-chave
Antimicrobial Antioxidant By-products Chitosan Chitosan nanoparticles Grapevines Phenolics
Contexto Educativo
Citação
Silva, Vanessa; Singh, Rupesh Kumar; Gomes, Nelson; Soares, Bruno Gonçalves; Silva, Adriana; Falco, Virgílio; Capita, Rosa; Alonso-Calleja, Carlos; Pereira, José Eduardo; Amaral, Joana S.; Igrejas, Gilberto; Poeta, Patrícia (2020). Comparative insight upon chitosan solution and chitosan nanoparticles application on the phenolic content, antioxidant and antimicrobial activities of individual grape components of Sousão variety. Antioxidants. ISSN 2076-3921. 9:2, p. 1-14