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Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke

2025Artigo científico Desconhecido
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorEsteves, Filipa
dc.contributor.authorMadureira, Joana
dc.contributor.authorBarros, Bela
dc.contributor.authorAlves, Sara
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorOliveira, Marta
dc.contributor.authorSlezakova, Klara
dc.contributor.authorFernandes, Adília
dc.contributor.authorPereira, Maria do Carmo
dc.contributor.authorMorais, Simone
dc.contributor.authorBonassi, Stefano
dc.contributor.authorTeixeira, João P.
dc.contributor.authorCosta, Solange
dc.date.accessioned2026-03-23T15:04:01Z
dc.date.available2026-03-23T15:04:01Z
dc.date.issued2025
dc.description.abstractOccupational exposure as a firefighter is classified as carcinogenic to humans. However, information on the biological effects of wildland firefighting remains limited. This study aimed to assess genotoxicity in a group of wildland firefighters and evaluate the contribution of total concentration of urinary hydroxylated polycyclic aromatic hydrocarbons (ΣOHPAHs) to selected endpoints. A group of 59 northern Portuguese wildland firefighters (mean age: 35.5 ± 9.0 years) was evaluated before and during the wildfire season. Sociodemographic, lifestyle, occupational-related information was collected via questionnaire. The micronucleus (MN) assay in buccal and urothelial cells was applied to paired samples to assess the association with exposure, as indicated by urinary ΣOHPAHs levels. The risk of MN formation in urothelial cells was over twofold higher during the wildfire season [Frequency Ratio (FR): 2.13, 95 % CI: 1.99–2.27, p = 0.01]. A 35 % increase in MN frequency in buccal cells was observed during the wildfire season (FR: 1.35, 95 % CI: 0.76–2.40, p > 0.05). Urinary ΣOHPAHs exhibited a significant positive association with MN‰ in urothelial cells (FR:1.04, 95 % CI: 1.01–1.08, p < 0.05). This study provides the first assessment of MN frequency in urothelial cells of wildland firefighters, offering novel evidence of genotoxic risks and potential long-term health impacts associated with wildland firefighting.eng
dc.description.sponsorshipThis work received financial support from the project PCIF/SSO/0017/2018 (https://doi.org/10.54499/PCIF/SSO/0017/2018) by the Fundação para a Ciência e a Tecnologia (FCT), Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), through national funds. This work is financed by national funds through the FCT within the scope of projects UIDB/04750/2020 (https://doi.org/10.54499/UIDB/04750/2020) and LA/P/0064/2020 (https://doi.org/10.54499/LA/P/0064/2020). Filipa Esteves was supported by National Funds through FCT, under the Ph.D. fellowship UI/BD/150783/2020. The work of Stefano Bonassi was supported by the Italian Ministry of Health (Ricerca Corrente), Rome, Italy, and by the Competitive Funding for University Research Projects [Finanziamento competitivo di progetti di ricerca di ateneo (FIN/RIC)] San Raffaele University, Rome, Italy.
dc.identifier.citationEsteves, Filipa; Madureira, Joana; Barros, Bela; Alves, Sara; Vaz, Josiana; Oliveira, Marta; Slezakova, Klara; Fernandes, Adília; Pereira, Maria do Carmo; Morais, Simone; Bonassi, Stefano; Teixeira, João P.; Costa, Solange (2025). Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire Smoke. Journal of Hazardous Materials. ISSN 0304-3894. 500, p. 1-9
dc.identifier.doi10.1016/j.jhazmat.2025.140427
dc.identifier.issn0304-3894
dc.identifier.urihttp://hdl.handle.net/10198/36224
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBioFirEx: Um painel de (bio)marcadores para a vigilância da saúde e da segurança do bombeiro
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relationLaboratory for Integrative and Translational Research in Population Health
dc.relation150783/2020
dc.relation.ispartofJournal of Hazardous Materials
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChromosomal damage
dc.subjectDNA damage
dc.subjectMicronucleus assay
dc.subjectWildfire smoke
dc.subjectWildland firefighters
dc.titleMicronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smokeeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPCIF/SSO/0017/2018
oaire.awardNumberUIDB/04750/2020
oaire.awardNumberLA/P/0064/2020
oaire.awardTitleBioFirEx: Um painel de (bio)marcadores para a vigilância da saúde e da segurança do bombeiro
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardTitleLaboratory for Integrative and Translational Research in Population Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PCIF%2FSSO%2F0017%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PT
oaire.citation.titleJournal of Hazardous Materials
oaire.citation.volume500
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameVaz
person.familyNameFernandes
person.givenNameJosiana A.
person.givenNameAdília
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id0514-76CD-8CCF
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0003-1658-4509
person.identifier.ridV-8399-2017
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id56489572200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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