Publicação
Dual-aptasensor array: optimization of the aptamers immobilization
| dc.contributor.author | Ezziddine, Maha | |
| dc.contributor.author | Meirinho, Sofia G. | |
| dc.contributor.author | Veloso, Ana C.A. | |
| dc.contributor.author | Dias, L.G. | |
| dc.contributor.author | Rodrigues, Lígia R. | |
| dc.contributor.author | Othmane, Ali | |
| dc.contributor.author | Peres, António M. | |
| dc.date.accessioned | 2020-02-27T10:33:47Z | |
| dc.date.available | 2020-02-27T10:33:47Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | In the present work it is intended to establish the optimal experimental conditions that allow enhancing the performance of an electrochemical dual-aptasensor array for the detection of osteopontin, which has been reported as a cancer biomarker. The aptamer concentration, time and temperature of immobilization onto dual-screen printed gold electrodes (Dual-SPGE), as well as the aptamer-protein interaction time were evaluated using a 2k factorial experimental design. The preliminary results allowed identifying the optimal experimental conditions, namely: (i) aptamer concentration of 0.5 μM for both DNA and RNA aptamers; (ii) incubation temperature of 4ºC; (iii) time for aptamer immobilization onto the Dual-SPGE at 4ºC of 20 min; and, (iv) aptamer-protein interaction time of 30 min. The evaluation of the best experimental conditions was carried out by cyclic and square wave voltammetry using a ferro/ferricyanide solution ([Fe(CN)6]3−/4−) as a redox probe. Aptamers were immobilized onto the Dual-SPGE via streptavidin-biotin interaction. With the preliminary optimal experimental conditions herein obtained, we will further assess the sensitivity of the Dual-SPGE using synthetic plasma. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Ezzedine, Maha; Meirinho, Sofia G.; Veloso, Ana C.A.; Dias, L.G.; Rodrigues, Lígia R.; Othmane, Ali; Peres, António M. (2017). Dual-aptasensor array: Optimization of the aptamers immobilization. In BIOLIVAL 2017, 8es Journées Scientifiques Internationales sur la Valorisation des Bioressources. Monastir, Tunisia | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/20711 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | Aptasensor | pt_PT |
| dc.subject | Aptamer immobilization | pt_PT |
| dc.title | Dual-aptasensor array: optimization of the aptamers immobilization | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Monastir, Tunisia | pt_PT |
| oaire.citation.title | BIOLIVAL 2017 | 8es Journées Scientifiques Internationales sur la Valorisation des Bioressources | pt_PT |
| person.familyName | Dias | |
| person.familyName | Peres | |
| person.givenName | Luís G. | |
| person.givenName | António M. | |
| person.identifier | 107333 | |
| person.identifier.ciencia-id | 2F11-9092-FAAF | |
| person.identifier.ciencia-id | CF16-5443-F420 | |
| person.identifier.orcid | 0000-0002-1210-4259 | |
| person.identifier.orcid | 0000-0001-6595-9165 | |
| person.identifier.rid | I-8470-2012 | |
| person.identifier.scopus-author-id | 23569169900 | |
| person.identifier.scopus-author-id | 7102331969 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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| relation.isAuthorOfPublication | 7d93be47-8dc4-4413-9304-5b978773d3bb | |
| relation.isAuthorOfPublication.latestForDiscovery | 7d93be47-8dc4-4413-9304-5b978773d3bb |