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Applicability of HRM analysis for carnaroli rice authentication based on polymorphisms of the waxy gene

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dc.contributor.authorGrazina, Liliana
dc.contributor.authorCosta, Joana
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorGarino, Cristiano
dc.contributor.authorArlorio, Marco
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorMafra, Isabel
dc.date.accessioned2020-06-09T09:01:44Z
dc.date.available2020-06-09T09:01:44Z
dc.date.issued2018
dc.description.abstractRice (Oryza sativa L.) is a staple food and one of the most important cereals in the worldwide. Italy, the leading rice producer in Europe, holds nearly 200 different varieties in the available germplosm [1]. The Carnaroli rice is a high quality and priced variety belonging to the group of ja ponica ecotype, produced mainly in Piedmont. it is considered one of the finest Italian rice varieties due to its excellent cooking resistance, given by a low tendency to lose starch and a good ability to absorb liquid while creaming, being, thus, ideal for the preparation of traditional risotto. Italian rice varieties hove different characteristics, from which the starch composition is a highly relevant parameter. Together with amylopectin, amylose is the main component of starch, whose ratio is determinant for the rice cooking properties. After cooking, varieties with high amylose content have dry, firm and separate groins, while low amylose ones usually hove tender, cohesive and glossy texture [2]. Amylose synthesis is catalysed by the granule bound starch synthase (GBSS) that is encoded by the Waxy gene (Wx), being located on the chromosome 6. Various nucleotide polymorphisms have been associated with the Wx gene, namely (CT)n repeats and several single nucleotide polymorphisms (SNP) [2]. The aim of this work was to propose a new method based on high resolution melting (HRM) analysis, exploiting those polymorphisms to differentiate Carnaroli rice from other closely related varieties.pt_PT
dc.description.sponsorshipThis work has been supported by the European project FOODINTEGRITY (FP7-KBBE- 2013-single-stage, No 613688), by FCT (Fundação para a Ciencia e Tecnologia) through project UID/QUI/50006/2013 - POCI/ 01/0145/FEDER/ 007265 with financial support from FCT /MEC through national funds and eo-financed by FED ER. under the Partnership Agreement PT2020 and by the project NORTE-01-0145-FEDER-000011. L. Grazina and J. Costa ore grateful to grants (SFRH/ BD/132462/2017 and SFRH/BPD/102404/2014, respectively) from FCT financed by POPH-QREN (subsidised by FSE and MCTES).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrazina, Liliana; Costa, J.; Amaral, J.S.; Garino, C.; Arlorio, M.; Oliveira, M.B.P.P.; Mafra, I. (2018). Applicability of HRManalysis for carnaroli rice authentication based on polymorphisms of the waxy gene. In 5th Food Integrity Conference. Nantes. ISBN 978-2-9566303-2-6pt_PT
dc.identifier.isbn978-2-9566303-2-6
dc.identifier.urihttp://hdl.handle.net/10198/22041
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationEnsuring the Integrity of the European food chain
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectHRM analysispt_PT
dc.subjectRicept_PT
dc.titleApplicability of HRM analysis for carnaroli rice authentication based on polymorphisms of the waxy genept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleEnsuring the Integrity of the European food chain
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/613688/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.conferencePlaceNantes, Francept_PT
oaire.citation.endPage211pt_PT
oaire.citation.startPage210pt_PT
oaire.citation.title5th Food Integrity Conferencept_PT
oaire.fundingStreamFP7
oaire.fundingStream5876
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isProjectOfPublication050e7d15-3b12-4aa1-b7a9-bd919b5696fb
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