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Antioxidant and hepatotoxic activities of gama irradiated borututu

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dc.contributor.authorPereira, Carla
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorAntonio, Amilcar L.
dc.contributor.authorQueiroz, Maria João R.P.
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2015-10-26T11:27:47Z
dc.date.available2015-10-26T11:27:47Z
dc.date.issued2014
dc.description.abstractBorututu is a well-known medicinal plant in Angola for the treatment of liver diseases. Our research group reported, in recent studies, that its infusion, pills and syrups display significant antioxidant and anti-hepatocellular carcinoma activities [1,2]. In order to preserve the safety of this medicinal plant, which can be compromised during the processing and storage period, gamma irradiation arises as a safe technology to avoid the contamination with pathogens in alternative to the traditional chemical fumigation. In the present study, we aimed to investigate the effect of different doses of gamma irradiation (1 and 10 kGy) on this plant regarding to the mentioned bioactive properties; therefore, infusions and methanolic extracts prepared from the irradiated plant were evaluated for their antioxidant activity (DPPH scavenging activity, reducing power, ~-carotene bleaching inhibition, and TBARS formation inhibition), anti-hepatocellular carcinoma activity (HepG2 tumor cell line) and hepatotoxicity (non-tumor liver cells primary culture). In a general way, the infusion and methanolic extract obtained from the sample irradiated at 10 kGy had a higher antioxidant activity in all the assays performed, with better results for the methanolic extract (EC50 values ranging from 0.04 and 0.24 mg/ml) when compared to the infusion (0.03-1.34 mg/ml). All the methanolic extracts revealed anti-hepatocellular carcinoma activity with a slightly decrease in the sample irradiated at 10 kGy (Gl50=188.97 J-19/ml) relatively to the control sample (GI50=160.02 1-Jg/ ml ). None of the infusions or methanolic extracts revealed toxicity in non-tumor porcine liver cells (GI50>400 1-Jg/ml). According to the results obtained for the antioxidant and antihepatocellular carcinoma activities, the risk of limiting these properties due to the irradiation at the tested doses can be excluded, making the gamma irradiation a suitable technique for borututu preservation.pt_PT
dc.identifier.citationPereira, Carla; Calhelha, Ricardo C.; Antonio, Amilcar L.; Queiroz, Maria João R.P.; Barros, Lillian; Ferreira, Isabel C.F.R. (2014). Antioxidant and hepatotoxic activities of gama irradiated borututu. In NUTECH 2014 - Development and Applications of Nuclear Technologies. Varsóvia, Polónia. ISBN 978-83-933935-2-7pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/12194
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationStrategic Project - UI 690 - 2011-2012
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleAntioxidant and hepatotoxic activities of gama irradiated borututupt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.citation.titleNUTECH-2014. Development and Applications of Nuclear Technologiespt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira
person.familyNameCalhelha
person.familyNameAntonio
person.familyNameBarros
person.familyNameFerreira
person.givenNameCarla
person.givenNameRicardo C.
person.givenNameAmilcar L.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier1415151
person.identifier469085
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person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0001-8271-9964
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridK-1629-2016
person.identifier.ridJ-2172-2014
person.identifier.ridJ-1960-2014
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id55627876424
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id44560921900
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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