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Phenolic composition and in vitro bioactivities of cardoon blades: study of its potential as a functional ingredient

2023Conference object Open access
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dc.contributor.authorMandim, Filipa
dc.contributor.authorPetropoulos, Spyridon Α.
dc.contributor.authorPinela, José
dc.contributor.authorDias, Maria Inês
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-11-16T12:13:48Z
dc.date.available2023-11-16T12:13:48Z
dc.date.issued2023
dc.description.abstractCynara cardunculus L. var. altilis DC., commonly known as cardoon, belongs to the Asteraceae family. This species is integrated into the Mediterranean diet and also has several industrial applications (e.g., pharmaceutical, cosmetic, energetic, paper pulp, and food industries) [1]. Over the last decade, cardoon has been attracting high interest from several industrial sectors. In addition to low requirements in agronomic inputs and high adaptation to adverse environmental conditions, cardoon exhibits a high concentration of bioactive compounds as well as biomass yields [1,2]. Despite the multifaceted applications of cardoon, vegetable tissues such as blades and floral stems are commonly discarded and consequently considered biowaste [1,3]. The adequate exploitation and characterization of the species are essential for circular economic stimulation and environmental impact reduction. Cardoon blades were collected in Greece at sixteen maturation stages (B1-B16). Phenolic composition and several in vitro bioactive properties of their hydroethanolic extracts were studied. The polyphenolic profile was analyzed by HPLC-DAD-ESI/MS. The antioxidant potential was studied through two cell-based assays: TBARS and OxHLIA. The anti- inflammatory activity was evaluated through the extracts’ capacity to inhibit the formation of the pro- inflammatory mediator nitric oxide. Finally, the antiproliferative potential was evaluated against four tumors and a non-tumor cell line (PLP2) using the sulforhodamine B colorimetric assay. Twenty phenolic compounds were tentatively identified. 5-O-Caffeoylquinic acid, trans 3,4-O-dicaffeoylquinic acid, and luteolin-O-hexoside were the compounds found in higher concentrations. Immature blades (B3) exhibited the highest phenolic content (178 mg/g extract) and capacity to inhibit the thiobarbituric acid reactive substances formation (IC50 = 1.61 g/mL). On the other hand, samples at more advanced stages of maturation exhibited higher antiproliferative (B8–B13, GI50 between 7 and 17 μg/mL) and anti- inflammatory activities (B13, IC50 of 10 μg/mL), as well as higher capacity to inhibit oxidative hemolysis (B8, IC50 = 25 and 47 μg/mL for ∆t of 60 and 120 min, respectively). This study proved that the maturity stage influences the phenolic composition and bioactive potential of cardoon blades which could be valorized as a functional ingredient for food and pharmaceutical applications.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021); PhD grant of Filipa Mandim (SFRH/146614/2019) and J. Pinela (CEECIND/01011/2018), M.I. Dias and L. Barros contractspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMandim, Filipa; Petropoulos, Spyridon Α.; Pinela, José; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Santos-Buelga, Celestino; Barros, Lillian (2023). Phenolic composition and in vitro bioactivities of cardoon blades: study of its potential as a functional ingrediente. In 8th Portuguese Young Chemistry Meeting. Vila Realpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/28889
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Portuguesa de Químicapt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCynara cardunculus L.pt_PT
dc.titlePhenolic composition and in vitro bioactivities of cardoon blades: study of its potential as a functional ingredientpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceVila Realpt_PT
oaire.citation.endPage83pt_PT
oaire.citation.startPage83pt_PT
oaire.citation.title8th Portuguese Young Chemistry Meetingpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMandim
person.familyNamePinela
person.familyNameDias
person.familyNameFerreira
person.familyNameBarros
person.givenNameFilipa
person.givenNameJosé
person.givenNameMaria Inês
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifier144781
person.identifier469085
person.identifier.ciencia-idF318-9049-EB99
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridB-4466-2014
person.identifier.ridM-8242-2013
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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