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A new effective assay to detect antimicrobial activity of filamentous fungi

2013Journal article Restricted access
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dc.contributor.authorPereira, Eric Carvalho
dc.contributor.authorSantos, A.
dc.contributor.authorReis, Francisca
dc.contributor.authorTavares, Rui Manuel
dc.contributor.authorBaptista, Paula
dc.contributor.authorLino-Neto, Teresa
dc.contributor.authorAlmeida-Aguiar, Cristina
dc.date.accessioned2014-09-19T14:46:27Z
dc.date.available2014-09-19T14:46:27Z
dc.date.issued2013
dc.description.abstractThe search for new antimicrobial compounds and the optimization of production methods turn the use of antimicrobial susceptibility tests a routine. The most frequently used methods are based on agar diffusion assays or on dilution in agar or broth. For filamentous fungi, the most common antimicrobial activity detection methods comprise the co-culture of two filamentous fungal strains or the use of fungal extracts to test against single-cell microorganisms. Here we report a rapid, effective and reproducible assay to detect fungal antimicrobial activity against single-cell microorganisms. This method allows an easy way of performing a fast antimicrobial screening of actively growing fungi directly against yeast. Because it makes use of an actively growing mycelium, this bioassay also provides a way for studying the production dynamics of antimicrobial compounds by filamentous fungi. The proposed assay is less time consuming and introduces the innovation of allowing the direct detection of fungal antimicrobial properties against single cell microorganisms without the prior isolation of the active substance(s). This is particularly useful when performing large screenings for fungal antimicrobial activity. With this bioassay, antimicrobial activity of Hypholoma fasciculare against yeast species was observed for the first time.por
dc.identifier.citationPereira, Eric Carvalho; Santos A.; Reis, Francisca; Tavares, Rui Manuel; Baptista, Paula; Lino-Neto, Teresa; Almeida-Aguiar, Cristina (2013). A new effective assay to detect antimicrobial activity of filamentous fungi. Microbiological Research. ISSN 0944-5013. 168:1, p. 1-5por
dc.identifier.doi10.1016/j.micres.2012.06.008
dc.identifier.issn0944-5013
dc.identifier.urihttp://hdl.handle.net/10198/10492
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationStrategic Project - UI 4050 - 2011-2012
dc.subjectAntimicrobial assaypor
dc.subjectAnti-yeast activitypor
dc.subjectFilamentous funguspor
dc.subjectMicrobiological methodpor
dc.titleA new effective assay to detect antimicrobial activity of filamentous fungipor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 4050 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F099556%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FBIA%2FUI4050%2F2011/PT
oaire.citation.endPage5por
oaire.citation.issue1por
oaire.citation.startPage1por
oaire.citation.titleMicrobiological Researchpor
oaire.citation.volume168por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
person.familyNameBaptista
person.givenNamePaula
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0001-6331-3731
person.identifier.scopus-author-id14051688000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isProjectOfPublication67dd9919-6192-4c28-a9bc-009afc09309f
relation.isProjectOfPublication35d9ef43-18e7-4e47-a5fc-8ff7581bccb4
relation.isProjectOfPublication.latestForDiscovery67dd9919-6192-4c28-a9bc-009afc09309f

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