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Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products

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dc.contributor.authorAmaral, Joana S.
dc.contributor.authorSantos, G.
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorMafra, Isabel
dc.date.accessioned2020-06-09T09:42:02Z
dc.date.available2020-06-09T09:42:02Z
dc.date.issued2017
dc.description.abstractThe development of highly sensitive and quantitative tools to identify undeclared pork meat is very important to authenticate processed meat products and, particularly, in the case of Halal products. Quantitative approaches are crucial to distinguish deliberate adulterations from cross-contaminations. This study intended to develop and validate a novel specific and highly sensitive Evagreen real-time PCR system for pork meat quantification in processed meat products. A normalised assay based on the ΔCt method was successfully developed and optimised, allowing the detection and quantification of levels down to 0.0001% and 0.01% (w/w) of pork meat, respectively, in both raw and thermally processed. The method was effectively validated using blind meat mixtures, exhibiting adequate parameters of trueness, precision and repeatability. Its application to several commercial samples, including Halal and regular meat products, showed the presence of undeclared pork species in 54% of the analysed samples, with 40% of Halal products presenting traces of pork, therefore not in good agreement with their label statements.pt_PT
dc.description.sponsorshipThis work was supported by FCT (Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013 e POCI/01/0145/ FEDER/007265 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and project NORTE-01-0145-FEDER-000011.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral, J.S.; Santos, G.; Oliveira, M.B.P.P.; Mafra, I. (2017). Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products. Food Control. ISSN 0956-7135. 72, p. 53-61pt_PT
dc.identifier.doi10.1016/j.foodcont.2016.07.029pt_PT
dc.identifier.issn0956-7135
dc.identifier.urihttp://hdl.handle.net/10198/22045
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectSus scrofapt_PT
dc.subjectMeat productspt_PT
dc.subjectHalal productspt_PT
dc.subjectSpecies detectionpt_PT
dc.subjectQuantificationpt_PT
dc.subjectReal-time PCRpt_PT
dc.titleQuantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage61pt_PT
oaire.citation.startPage53pt_PT
oaire.citation.titleFood Controlpt_PT
oaire.citation.volume72pt_PT
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd

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