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Photoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue – DMMB and laser light

2025Journal article Open access
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dc.contributor.authorSantos, Gustavo Vital dos
dc.contributor.authorMoura, Hesrom Fernandes Serra
dc.contributor.authorCrugeira, Pedro Jorge Louro
dc.contributor.authorSilva, Anna Paula Lima Teixeira da
dc.contributor.authorCastro, Isabele Cardoso Vieira de
dc.contributor.authorCosta, Wellington Luís Reis
dc.contributor.authorAlmeida, Paulo Fernando de
dc.contributor.authorPinheiro, Antonio Luiz Barbosa
dc.date.accessioned2025-02-10T11:11:51Z
dc.date.available2025-02-10T11:11:51Z
dc.date.issued2025
dc.description.abstractAnnually, the oil and gas industry faces equipment losses and product quality degradation due to the presence of sulfate-reducing bacteria (SRB). Given the negative impact of SRB, this study evaluates the use of photoinactivation (PI) with zinc chloride double salt of 1,9-Dimethyl-Methylene Blue (DMMB) as a photosensitizer (PS) in varying concentrations and combined with Laser light at different exposures in an SRB consortium. For culture growth, a modified Postgate C medium (without ferrous sulfate) was used, and cell quantification was performed on 100 μL aliquots of the consortium, read on a spectrophotometer (λ600 nm) in an oxygen- and lightfree environment at room temperature. Statistical analyses included two-way ANOVA and ANOVA with interaction to separately and jointly evaluate the effects of PS and light in PI. Results indicated microbial activity in all groups, with an antimicrobial inhibition rate exceeding 50 % (p < 0.05) for concentrations above 1.5 μg/mL of DMMB. PI efficacy significantly depended on DMMB concentration and light density, achieving a 70.58 % (55.73–70.58, with a mean of 66.71 %) reduction (p < 0.05) with 1.5 μg/mL of DMMB and a 70.15 % (65–70.15, with a mean of 68.21 %) reduction with 2.0 μg/mL at an intensity of 21.6 J/cm2. In conclusion, PI presents a promising alternative to biocides in the oil and gas industry, offering easy application, avoiding bacterial resistance, being environmentally safe, and compatible with other SRB population control techniques.pt_PT
dc.description.sponsorshipAuthors G. V. dos Santos thank the Fundação de Amparo a Pesquisa do Estado da Bahia (Fabesb) for an MSci Grant (BOL0426/2021) and A. P. L.T. da Silva for a PhD Grant (BOL0667/2021). Authors P. F. de Almeida and A. L. B. Pinheiro would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research grants (respectively 302753/2020-6 and 304279/2018-8).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, Gustavo Vital dos; Moura, Hesrom Fernandes Serra; Crugeira, Pedro Jorge Louro; Silva, Anna Paula Lima Teixeira da; Castro, Isabele Cardoso Vieira de; Costa, Wellington Luís Reis; Almeida, Paulo Fernando de; Pinheiro, Antonio Luiz Barbosa (2025). Photoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue – DMMB and laser light. Journal of Photochemistry and Photobiology B: Biology. ISSN 1011-1344. 263, p. 1-9pt_PT
dc.identifier.doi10.1016/j.jphotobiol.2025.113103pt_PT
dc.identifier.issn1011-1344
dc.identifier.urihttp://hdl.handle.net/10198/31133
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectBiocidespt_PT
dc.subjectLaserpt_PT
dc.subjectPhotoinactivationpt_PT
dc.subjectSRBpt_PT
dc.titlePhotoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue – DMMB and laser lightpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Photochemistry and Photobiology B: Biologypt_PT
oaire.citation.volume263pt_PT
person.familyNameCrugeira
person.givenNamePedro
person.identifier.ciencia-id8F17-E60C-A45D
person.identifier.orcid0000-0002-7084-3711
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationce777646-e21d-4491-a8a8-345efc9517a1
relation.isAuthorOfPublication.latestForDiscoveryce777646-e21d-4491-a8a8-345efc9517a1

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