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Anthocyanin composition and bioactivities of the hydroethanolic extract of Garcinia mangostana L. pericarp

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dc.contributor.authorAlbuquerque, Bianca R.
dc.contributor.authorDias, Maria Inês
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorPinela, José
dc.contributor.authorBarros, Lillian
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2022-02-01T15:26:12Z
dc.date.available2022-02-01T15:26:12Z
dc.date.issued2021
dc.description.abstractGarcinia mangostana L., commonly known as mangosteen or queen of fruit, is a tropical fruit belonging to the Clusiaceae family. This shrub is native from South Asia, but can also be found in other tropical territories, such as South America [1,2,3]. The fruit comprises an inedible dark purple or red pericarp (> 60%) that encases the edible succulent pulp [2]. Nowadays, mangosteen pulp and pericarp have been used in beverages as a food supplement by virtue of the traditional knowledge about its benefits. However, a poor correlation between the medicinal use of mangosteen and its chemical composition has been described [2,3]. Aiming to contribute to the elucidation of the phytochemical composition of this fruit pericarp, the present study was carried out to characterize anthocyanins by HPLC-DAD-ESI/MS. In addition, the bioactivities of the hydroethanolic extract obtained by solid-liquid extraction were evaluated. The extract cytotoxicity to four human tumour cell lines (NCI-H460 - lung carcinoma, MCF-7 - breast carcinoma, HepG2 - hepatocellular carcinoma, and HeLa - cervical carcinoma) was tested by the sulforhodamine B assay. The anti-inflammatory activity was evaluated by the ability to inhibit the lipopolysaccharide-induced nitric oxide (NO) production by murine macrophage RAW 264.7 cells. Lastly, the antioxidant activity was measured by the extract capacity to inhibit the oxidative haemolysis (OxHLIA) and the formation of thiobarbituric acid reactive species (TBARS), using sheep erythrocytes and porcine brain cells as oxidizable substrates. The chromatographic analysis allowed the identification of two anthocyanins in the mangosteen pericarp, namely cyanidin-O-dihexoside, which was the most abundant, and delphinidin-O-pentoside. Regarding the in vitro evaluated bioactive properties, the extract showed cytotoxic activity against the four human tumour cell lines, with GI50 values lower than 75 μg/mL. It also exhibited antioxidant and anti-inflammatory activities at low concentrations, with IC50 values of 8.7 and 17.3 μg/mL for the TBARS and OxHLIA assays, and an EC50 value of 85 μg/mL for the NO production inhibition assay, respectively. The results obtained in this study allow to conclude that G. mangostana pericarp contains an interesting amount of anthocyanins and also exhibits strong bioactivities, which makes it an interesting source of bioactive ingredients.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020). M.I. Dias, J. Pinela, R.C. Calhelha and L. Barros thank their research contracts (national funding by FCT, P.I., through the institutional scientific employment program-contract) and B.R. Albuquerque thanks her PhD grant (SFRH/BD/136370/2018). This work was funded by the FEDER through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE and project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®. To FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlbuquerque, Bianca R.; Dias, Maria Inês; Calhelha, Ricardo; Pinela, José; Barros, Lillian; Oliveira, Beatriz; Ferreira, Isabel C.F.R. (2021). Anthocyanin composition and bioactivities of the hydroethanolic extract of Garcinia mangostana L. Pericarp. In 7th Portuguese Young Chemists Meeting. ISBN 978-989-8124-31-0pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/24989
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationNatural colorants based on anthocyanins obtained from bioresidues
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGarcinia mangostana L.pt_PT
dc.subjectTropical fruitpt_PT
dc.titleAnthocyanin composition and bioactivities of the hydroethanolic extract of Garcinia mangostana L. pericarppt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberSFRH/BD/136370/2018
oaire.awardTitleMountain Research Center
oaire.awardTitleNatural colorants based on anthocyanins obtained from bioresidues
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F136370%2F2018/PT
oaire.citation.conferencePlaceonline editionpt_PT
oaire.citation.title7th Portuguese Young Chemists Meetingpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlbuquerque
person.familyNameDias
person.familyNameCalhelha
person.familyNamePinela
person.familyNameBarros
person.familyNameFerreira
person.givenNameBianca R.
person.givenNameMaria Inês
person.givenNameRicardo C.
person.givenNameJosé
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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