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Diagnosis of ink disease of chestnut by molecular identification of associated phytophthora species

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dc.contributor.authorGouveia, Maria Eugénia
dc.contributor.authorCoelho, Valentim
dc.contributor.authorChoupina, Altino
dc.contributor.authorAbreu, Carlos Gomes
dc.contributor.authorHermosa, R.
dc.contributor.authorMonte, Enrique
dc.date.accessioned2014-10-21T09:44:38Z
dc.date.available2014-10-21T09:44:38Z
dc.date.issued2005
dc.description.abstractFor diagnostic proposes of ink disease, chestnut orchards with symptoms of decline or sudden death of trees were sampled by soil baiting techniques and selective agar media (P10VPH). Thirty-six Phytophthora isolates were obtained. One isolate per tree and three or two isolates from the soil of the same plant were considered for molecular identification. Genomic DNA was extracted from all the isolates and from the reference strain P. cinnamomi CECT 2965. The ribosomal regions ITS1, 5.8S and ITS2 were amplified with the universal primer pair ITS6 (Cooke and Duncan, 1997) and ITS4 (White et al., 1990) by PCR. The amplified fragment (900 pb) was digested with restriction enzymes MspI, AluI and TaqI. Two different patterns of fingerprinting were obtained with enzymes TaqI and AluI (type I and II) and three different patterns with MspI (type I, Ia, II). The fingerprinting of each isolate was compared with database of CABI by public web access. Type I and Ia (14 isolates) were assigned to P. cinnamomi and type II (4 isolates) was assigned to P. cambivora. Molecular methods provide a rapid means of Phytophthora species identification associated with ink disease of chestnut and will provide a useful tool for etiological and epidemiological studies of this important disease of chestnut.por
dc.identifier.citationGouveia, M.E., Coelho, V., Choupina, A., Abreu, Carlos, Hermosa, R.; Monte, E. (2005). Diagnosis of ink disease of chestnut by molecular identification of associated phytophthora species. In 3rd International Chestnut Congress. Chaves. Acta Horticulturae. ISSN 0567-7572. p. 585-590por
dc.identifier.doi10.17660/ActaHortic.2005.693.77
dc.identifier.issn0567-7572
dc.identifier.urihttp://hdl.handle.net/10198/10926
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectP. cinnamomipor
dc.subjectP. cambivorapor
dc.subjectPhytophthora detectionpor
dc.subjectITS-RFLP identificationpor
dc.titleDiagnosis of ink disease of chestnut by molecular identification of associated phytophthora speciespor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortugalpor
oaire.citation.endPage589por
oaire.citation.issue693por
oaire.citation.startPage585por
oaire.citation.titleActa Horticulturaepor
person.familyNameGouveia
person.familyNameCoelho
person.familyNameChoupina
person.givenNameMaria Eugénia
person.givenNameValentim
person.givenNameAltino
person.identifier587972
person.identifier.ciencia-id0618-49BC-EACE
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-2550-9108
person.identifier.orcid0000-0001-5829-7669
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id26533798200
person.identifier.scopus-author-id14051602500
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication99e4701b-e3c2-4594-a37f-42bd49821d6e
relation.isAuthorOfPublication99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication.latestForDiscovery99e4701b-e3c2-4594-a37f-42bd49821d6e

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