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Exploring the α-amylase-inhibitory properties of tannin-rich extracts of Cytinus hypocistis on starch digestion

2023Journal article Restricted access
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dc.contributor.authorCorrea, Vanesa G.
dc.contributor.authorGarcia-Manieri, Jéssica A.A.
dc.contributor.authorSilva, Ana Rita
dc.contributor.authorBackes, Emanueli
dc.contributor.authorCorrêa, Rúbia C.G.
dc.contributor.authorBarros, Lillian
dc.contributor.authorBracht, Adelar
dc.contributor.authorPeralta, Rosane M.
dc.date.accessioned2023-12-18T09:53:27Z
dc.date.available2023-12-18T09:53:27Z
dc.date.issued2023
dc.description.abstractCytinus hypocistis (L.) L. is an edible parasitic plant that grows within the roots of its host. In addition to its use as famine food in the past, it is also tradidionally used for treating several illnesses such as intestinal problems, inflammations, tumors, and bleeding. This species is rich in hydrolysable tannins, compounds often associated with inhibiting starch digestion. Therefore, the present work investigated how effectively C. hypocistis tannin-rich extracts inhibited enzymes involved in starch digestion and if such effect also occurs in vivo. The latter premise was approached using the starch tolerance test in mice. Two optimized hydroethanolic extracts were used, a heat-assisted and an ultrasound-assisted extract, with known hydrolysable tannin content. Both extracts demonstrated potent inhibition of α-amylase. Inhibitions were of the mixed type with inhibitor constants in the 15 μg/mL range. The inhibition of the intestinal α-glucosidase was at least ten times less effective. The inhibition of the α-amylase was negatively affected by in vitro gastrointestinal digestion and bovine serum albumin. In vivo, both extracts inhibited starch digestion at doses between 100 and 400 mg/mL in healthy mice. The highest doses of the ultrasound and heat extracts diminished the peak glucose levels in the starch tolerance test by 46 and 59.3%, respectively. In streptozotocin diabetic mice, this inhibition occurred only at the dose of 400 mg/mL. Under this condition, diminution of the peak glucose concentration in the starch tolerance test was equal to 36.7% and 48.8% for the ultrasound and heat extracts, respectively. Maltose digestion was not inhibited by the C. hypocistis extracts. Qualitatively and quantitatively, thus, the actions of both extracts were similar. The results allow adding a new biological property to C. hypocistis, namely, the ability to decrease the hyper-glycemic excursion after a starch-rich meal, propitiating at the same time a diminished caloric intake.pt_PT
dc.description.sponsorshipThis research was financed by Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) and Fundaç˜ao Arauc´aria. The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/ 0007/2020). A. R. Silva is grateful to FCT and FSE for her Doctoral Grant (SFRH/BD/145834/2019), and L. Barros for her institutional scientific employment program-contract. R. C. G. Corrêa is a research grant recipient of Cesumar Institute of Science, Technology, and Innovation (ICETI).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCorrea, Vanesa G.; Garcia-Manieri, Jéssica A.A.; Silva, Ana Rita; Backes, Emanueli; Corrêa, Rúbia C.G.; Barros, Lillian; Bracht, Adelar; Peralta, Rosane M. (2023). Exploring the α-amylase-inhibitory properties of tannin-rich extracts of Cytinus hypocistis on starch digestion. Food Research International. ISSN 0963-9969. 173, p. 1-12pt_PT
dc.identifier.doi10.1016/j.foodres.2023.113260pt_PT
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/28960
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relationCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDiabetespt_PT
dc.subjectHydrolysable tanninspt_PT
dc.subjectα-amylase inhibitionpt_PT
dc.subjectGastrointestinal digestionpt_PT
dc.titleExploring the α-amylase-inhibitory properties of tannin-rich extracts of Cytinus hypocistis on starch digestionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardTitleCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145834%2F2019/PT
oaire.citation.endPage12pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume173pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameSilva
person.familyNameBackes
person.familyNameCorrêa
person.familyNameBarros
person.givenNameAna Rita
person.givenNameEmanueli
person.givenNameRúbia C.G.
person.givenNameLillian
person.identifierABD-5857-2020
person.identifier469085
person.identifier.ciencia-id0E1A-35C1-C79A
person.identifier.ciencia-id6C1F-2818-CBB5
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0003-2875-9097
person.identifier.orcid0000-0003-0269-4344
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id37661286600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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