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Suillus luteus methanolic extract inhibits cell growth and proliferation of a colon cancer cell line

2013Journal article Restricted access
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dc.contributor.authorSantos, Tiago
dc.contributor.authorTavares, Catarina
dc.contributor.authorSousa, Diana
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorMartins, Anabela
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2013-07-24T13:35:25Z
dc.date.available2013-07-24T13:35:25Z
dc.date.issued2013
dc.description.abstractSeveral edible mushrooms extracts are known to have tumour cell growth inhibitory potential. The objective of this work was to study this potential in extracts of Suillus luteus collected from the Northeast of Portugal. Various extracts were prepared and their effect on tumour cell growth was studied with the SRB assay in four human tumour cell lines: MCF-7 (breast), NCI-H460 (non-small cell lung cancer), AGS (gastric) and HCT-15 (colon). The methanolic extract of S. luteus was the most potent extract. This extract was slightly more potent in the HCT-15 cells (with mutant p53, GI50=17.8 ± 1.6 µg/mL) than in the other cell lines tested, which suggested that its effect was not p53-dependent. Indeed, in HCT-15 cells, an increase in the levels of p53 was detected but no alterations in some of the proteins transactivated by p53 (e.g. p21 or Bax) were found. The extract caused an increase in the cellular levels of p-H2A.X, which is suggestive of DNA damage. Growth inhibition in these cells was mostly due to inhibition of cell proliferation and an increase in the % of cell in the G1 phase of the cell cycle. An increase in cell death was also found, even though to very low levels. In addition, this extract was not cytotoxic to primary cultures of porcine hepatocytes (GI50>400 µg/mL). Together, these results indicate that the S. luteus methanolic extract may be an interesting source of compounds that inhibit the proliferation of tumour cells but further studies should be carried out in order to understand its potential.por
dc.identifier.citationSantos, Tiago; Tavares, Catarina; Sousa, Diana; Vaz, Josiana A.; Calhelha, Ricardo C.; Martins, Anabela; Almeida, Gabriela M.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2013). Suillus luteus methanolic extract inhibits cell growth and proliferation of a colon cancer cell line. Food Research International. ISSN 0963-9969. 53:1, p. 476–481por
dc.identifier.doi10.1016/j.foodres.2013.05.037
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/8551
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBD/43653/2008
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationStrategic Project - UI 690 - 2011-2012
dc.subjectSuillus luteuspor
dc.subjectMethanolic extractpor
dc.subjectTumour cell growthpor
dc.subjectTumour cell proliferationpor
dc.titleSuillus luteus methanolic extract inhibits cell growth and proliferation of a colon cancer cell linepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.citation.endPage481por
oaire.citation.startPage476por
oaire.citation.titleFood Research Internationalpor
oaire.citation.volume53por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVaz
person.familyNameCalhelha
person.familyNameMartins
person.familyNameFerreira
person.givenNameJosiana A.
person.givenNameRicardo C.
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
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person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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