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The effect of a tannin enriched topical formulation on mice ears fungal biodiversity

2021Conference object Open access
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dc.contributor.authorSilva, Ana Rita
dc.contributor.authorPereira, Rebeca
dc.contributor.authorSoares, Ana Sofia
dc.contributor.authorCoelho, Ana Cláudia
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorOliveira, Paula A.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-01-31T11:17:45Z
dc.date.available2022-01-31T11:17:45Z
dc.date.issued2021
dc.description.abstractCytinus hypocistis (L.) L. is a holoparasitic plant on various Cistaceae family members occurring in the Mediterranean region. This specie has long been used in European popular medicine due to their astringent and haemostatic properties. Current studies attributed its antioxidant, anti-inflammatory, and antimicrobial properties to their hydrolysable tannin content. Therefore, the extracts of these plants might be a natural alternative treatment for many skin conditions. The main objective of the present work was to study the effect of C. hypocistis enriched topical formulation (EF) in mice ears fungal isolates using routine mycological techniques [1]. Three EF were prepared with different concentrations of C. hypocistis extracts: a) 3.1 mg extract (E)/g of cream; b) 6.2 mg E/g of cream; and c) 12.4 mg E/g of cream. The formulations were carefully mixed to guarantee sample homogeneity. A negative control (base cream) and the three EF were stored at 4 ºC during the experiments. Thirty samples were collected in the ears of 30 female mice. The mice were divided in six groups of 5 mice each, to evaluate the correct dosage and toxicity of the extract for the mice, although these were accessed in another essay. Two of the groups were of wild type, one was treated with the base cream (G6) and the other with the cream with highest concentration of extract (G4), and the four others of Papillomavirus models, a positive control on which was applied the base cream (G5) and rest were treated with three creams with rising concentrations of the extract (G1, G2 and G3, respectively). The experiment procedure was approved by a Animal Ethics Committee-DGAV number 0421/000/000/2014, 24/09/2014 (020172). Before the application, the surface of the ear was rubbed for 1 minute, using a swab and saline solution. The samples were transferred to Petri dishes with the culture medium Potato Dextrose Agar and the microscopic identification was carried out using the Lactophenol with Cotton Blue technique for staining the filamentous fungi and identification of the genera. In the entirety of samples collected on the first collection, 4 filamentous fungal genera were isolated: Penicillium (16.7%) in G1, G4 and G5, Mucor (16.7%) G1, G2 , G4 and in G5, Cladosporium (3.33%) in G1, Aspergillus (3.33%) in G6. In total, fungi were isolated in 14 (46.7%) animals. Fungal genera were not identified in three mice from G1 and G5. The remaining samples exhibiting either other types of microorganisms or no growth at all. After the application of C. hypocistis cream for 17 days in the ears, only one fungal colony was isolated in a mouse from G4. The genus was not identified. The results obtained suggest that C. hypocistis reduces the presence of fungal in the ears of mice.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). A. R. Silva is grateful to FCT for her Doctoral Grant (SFRH/BD/145834/2019) and L. Barros for her contract through the institutional scientific employment program. This work is funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project GreenHealth - Norte- 01-0145-FEDER-0pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Ana Rita; Pereira, Rebeca; Soares, Ana Sofia; Coelho, Ana Cláudia; Ferreira, Isabel C.F.R.; Oliveira, Paula A.; Barros, Lillian (2021). The effect of a tannin enriched topical formulation on mice ears fungal biodiversity. In 1st Natural products application: health, cosmetic and food: book of abstracts. Bragança: Instituto Politécnico. ISBN 978-972-745-286-6pt_PT
dc.identifier.isbn978-972-745-286-6
dc.identifier.urihttp://hdl.handle.net/10198/24961
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCytinus hypocistis (L.)pt_PT
dc.titleThe effect of a tannin enriched topical formulation on mice ears fungal biodiversitypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145834%2F2019/PT
oaire.citation.conferencePlaceonline editionpt_PT
oaire.citation.titleNatural products application: Health, Cosmetic, and Foodpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameSilva
person.familyNameFerreira
person.familyNameBarros
person.givenNameAna Rita
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifierABD-5857-2020
person.identifier144781
person.identifier469085
person.identifier.ciencia-id0E1A-35C1-C79A
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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