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Phenolic composition and bioactive properties of cardoon bracts: Influence of the growth cycle

2021Conference object Open access
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dc.contributor.authorMandim, Filipa
dc.contributor.authorPetropoulos, Spyridon Α.
dc.contributor.authorPinela, José
dc.contributor.authorDias, Maria Inês
dc.contributor.authorKostić, Marina
dc.contributor.authorSoković, Marina
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-01-20T15:05:45Z
dc.date.available2022-01-20T15:05:45Z
dc.date.issued2021
dc.description.abstractCynara cardunculus L. (cardoon) is a typical Mediterranean species that comprises the ancestor wild cardoon (var. sylvestris), the cultivated leafy cardoon (var. altilis) and globe artichoke (var. scolymus). It can be found worldwide due to its high adaptability to diverse climate conditions (e.g., resistance to extreme temperatures, water stress, and soils with variable pH) [1,2]. It is also considered a multipurpose crop due to its nutritional, pharmacological and industrial applications [2]. Due to the increase of its commercial and economic value, this study aimed to evaluate the polyphenolic composition and bioactive potential of cardoon bracts in relation to plant growth cycle. Cardoon bracts were collected in central Greece at eight different maturation stages (samples C1 – lower maturity to C8 – higher maturity). The phenolic profile was determined by HPLC-DAD-ESI/MS. The antioxidant activity was measured through two cell-based assays: TBARS (thiobarbituric acid reactive substances formation inhibition) and OxHLIA (oxidative hemolysis inhibition). Cytotoxic effects were screened against four human tumor cell lines and hepatotoxicity against a non-tumor cell line (PLP2) by the sulforhodamine B assay. The anti-inflammatory potential was tested through the inhibition of NO production by a murine macrophage cell line (RAW 264.7). Finally, the antibacterial and antifungal activities were evaluated by the broth microdilution method. Twelve phenolic compounds were tentatively identified in the cardoon bract extracts and quantified in higher amounts in immature samples. Immature bracts (C1) also revealed the highest cytotoxic (GI50 of 30 – 79 μg/mL) and anti-inflammatory (IC50 = 72 μg/mL) activities, while they presented the highest capacity to efficiently inhibit the formation of TBARS (IC50 = 26.8 μg/mL). In contrast, the extract with the higher maturity grade (C7) revealed superior efficacy against oxidative hemolysis (IC50 of 38 and 75 μg/mL at Δt=60 min and 120 min, respectively). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 and samples C2 and C4, respectively. The results obtained in this work could be helpful to choose the best harvesting time of cardoon bracts, allowing obtaining a greater variety of phenolic compounds, and consequently, a higher bioactive potential, and ultimately the most appropriate use of their constituents. Nevertheless, further studies are needed to better understand the compounds responsible for the observed activities, as well as to reveal the mechanisms involved in these activities.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); for the F. Mandim PhD grant (SFRH/BD/146614/2019), and the J. Pinela (CEECIND/01011/2018) and M.I. Dias and L. Barros contracts through the individual and institutional scientific employment program-contract, respectively. Also, to FEDER-Interreg España-Portugal programme for financial support through the projects 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMandim, Filipa; Petropoulos, Spyridon A.; Pinela, José; Dias, Maria Inês; Kostic, Marina; Sokovic, Marina; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Phenolic composition and bioactive properties of cardoon bracts: Influence of the growth cycle. In st Natural products application: health, cosmetic and food: book of abstracts. Bragança: Instituto Politécnico. ISBN 978-972-745-286-6pt_PT
dc.identifier.isbn978-972-745-286-6
dc.identifier.urihttp://hdl.handle.net/10198/24799
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCEECIND/01011/2018pt_PT
dc.relationMountain Research Center
dc.relationIntegrated strategies for the development of a novel functional beverage with sustainable production systems
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCardoon bractspt_PT
dc.subjectBioactive propertiespt_PT
dc.titlePhenolic composition and bioactive properties of cardoon bracts: Influence of the growth cyclept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleIntegrated strategies for the development of a novel functional beverage with sustainable production systems
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F146614%2F2019/PT
oaire.citation.titleNatural Products Application Healthpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMandim
person.familyNamePinela
person.familyNameDias
person.familyNameFerreira
person.familyNameBarros
person.givenNameFilipa
person.givenNameJosé
person.givenNameMaria Inês
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifier144781
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person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridB-4466-2014
person.identifier.ridM-8242-2013
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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