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Standard methods for pollen research

2021Journal article Open access
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dc.contributor.authorCampos, Maria G.
dc.contributor.authorAnjos, Ofélia
dc.contributor.authorChica, Manuel
dc.contributor.authorCampoy, Pascual
dc.contributor.authorNozkova, Janka
dc.contributor.authorAlmaraz-Abarca, Norma
dc.contributor.authorBarreto, Lidia M.R.C.
dc.contributor.authorNordi, João Carlos
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorPascoal, Ananias
dc.contributor.authorPaula, Vanessa B.
dc.contributor.authorChoupina, Altino
dc.contributor.authorDias, L.G.
dc.contributor.authorTešić, Živoslav L. j.
dc.contributor.authorMosić, Mirjana D.
dc.contributor.authorKostić, Aleksandar Ž.
dc.contributor.authorPešić, Mirjana B.
dc.contributor.authorMilojković-Opsenica, Dušanka M.
dc.contributor.authorSickel, Wiebke
dc.contributor.authorAnkenbrand, Markus J.
dc.contributor.authorGrimmer, Gudrun
dc.contributor.authorSteffan-Dewenter, Ingolf
dc.contributor.authorKeller, Alexander
dc.contributor.authorFörster, Frank
dc.contributor.authorTananaki, Chrysoula H.
dc.contributor.authorLiolios, Vasilios
dc.contributor.authorKanelis, Dimitrios
dc.contributor.authorRodopoulou, Maria-Anna
dc.contributor.authorThrasyvoulou, Andreas
dc.contributor.authorPaulo, Luísa
dc.contributor.authorKast, Christina
dc.contributor.authorLucchetti, Matteo A.
dc.contributor.authorGlauser, Gaëtan
dc.contributor.authorLokutova, Olena
dc.contributor.authorAlmeida-Muradian, Ligia Bicudo
dc.contributor.authorSzczęsna, Teresa
dc.contributor.authorCarreck, Norman L.
dc.date.accessioned2021-10-12T15:03:55Z
dc.date.available2021-10-12T15:03:55Z
dc.date.issued2021
dc.description.abstract“Bee pollen” is pollen collected from flowers by honey bees. It is used by the bees to nourish themselves, mainly by providing royal jelly and brood food, but it is also used for human nutrition. For the latter purpose, it is collected at the hive entrance as pellets that the bees bring to the hive. Bee pollen has diverse bioactivities, and thus has been used as a health food, and even as medication in some countries. In this paper, we provide standard methods for carrying out research on bee pollen. First, we introduce a method for the production and storage of bee pollen which assures quality of the product. Routine methods are then provided for the identification of the pollen’s floral sources, and determination of the more important quality criteria such as water content and content of proteins, carbohydrates, fatty acids, vitamins, alkaloids, phenolic and polyphenolic compounds. Finally, methods are described for the determination of some important bioactivities of bee pollen such as its antioxidant, anti-inflammatory, antimicrobial and antimutagenic properties. Métodos estándar Para la investigación del polen El "polen de abeja" es el polen recogido de las flores por las abejas melíferas. El polen de abeja es utilizado para nutrir a las propias abejas, principalmente para proporcionar jalea real y alimento para las crías, pero también se utiliza para la nutrición humana. Para este último fin, se recoge en la entrada de la colmena en forma de gránulos que las abejas llevan a la colmena. El polen de abeja tiene diversas bioactividades, por lo que se hautilizado como alimento para la salud, e incluso como medicamento en algunos países. En este artículo, proporcionamos métodos estándar para llevar a cabo investigaciones sobre el polen de abeja. En primer lugar, presentamos un método de producción y almacenamiento de polen de abeja que garantiza la calidad del producto. A continuación, se ofrecen métodos de rutina para la identificación de las fuentes florales del polen y la determinación de los criterios de calidad más importantes, como el contenido de agua y de proteínas, carbohidratos, ácidos grasos, vitaminas, alcaloides y compuestos fenólicos y polifenólicos. Por último, se describen métodos para la determinación de algunas bioactividades importantes del polen de abeja, como sus propiedades antioxidantes, antiinflamatorias, antimicrobianas y antimutagénicas.pt_PT
dc.description.sponsorshipThe COLOSS (Prevention of honey bee COlony LOSSes) Association aims to explain and prevent massive honey bee colony losses. It was funded through the COST Action FA0803. COST (European Cooperation in Science and Technology) is a unique means for European researchers to jointly develop their own ideas and new initiatives across all scientific disciplines through trans-European networking of nationally funded research activities. Based on a pan-European intergovernmental framework for cooperation in science and technology, COST has contributed since its creation more than 40 years ago to closing the gap between science, policy makers and society throughout Europe and beyond. COST is supported by the EU Seventh Framework Program for research, technological development and demonstration activities (Official Journal L 412, 30 December 2006). The European Science Foundation as implementing agent of COST provides the COST Office through an EC Grant Agreement. The Council of the European Union provides the COST Secretariat. The COLOSS network is now supported by the Ricola Foundation – Nature & Culture. Figures 26–28 are reproduced from Sawyer (1981) with the permission of the publishers University College Cardiff Press and Northern Bee Books. Lidia Barreto and J Nordi wish to thank the Apiculture Research Center of Taubate University (UNITAU-SP/Brazil) and Agriculture Secretary of Bahia State (SEAGRI-BA/ BRAZIL). Maria Campos wishes to thank (UI0204): UIDB/ 00313/2020, Center of Chemistry from Faculty of Sciences and Technology of University of Coimbra, Portugal. Of elia Anjos wishes to thank to Forest Research Centre, a research unit funded by Fundac¸~ao para a Ci^encia e a Tecnologia I.P. (FCT), Portugal (UIDB/00239/2020), and to Centro de Biotecnologia de Plantas da Beira Interior for the OPUS software availability. Norma Almaraz Abarca thanks to the Instituto Politecnico Nacional for financial and logistic support. Manuel Chica and Pascual Campoy wish to thank the APIFRESH project. APIFRESH has been co-funded by the European Commission under the R4SMEs 7th Framework Program. Olena Lokutova thanks the Austrian Institute of beekeeping, Dr H Pehhacker and the same members of the Institute H Hagel and E H€uttinger for conducting photomicroscopic studies and pollen analysis Ukrainian samples of pollen loads, which were the basis of the atlas of pollen “honey plants” of Ukraine. Olena is grateful also to Polish colleagues Z Warakomska (Department of Botany University of Lublin) and D Teper (Polish Institute of beekeeping) for their professionalism and consultations to determine the botanical origin of some Ukrainian honey. Also thanks to their scientific advisers’ academician G Bogdanov (National Academy of Agrarian Sciences of Ukraine), Prof. V Polishuk (Department of beekeeping National University of Life and Environmental Sciences of Ukraine) and O Martynyuk (M.G. Kholodny Institute of Botany, Kiev, Ukraine) for his helpful co-operation in the field of beekeeping and palinology. Janka Nozkova wishes to thank the Operational Program Research and Development of the European Regional Development Fund in the frame of the project “Support of technologies innovation for special bio-food products for human healthy nutrition” (ITMS 26220220115) and also by the Excellence Center for Agrobiodiversity Conservation and Benefit – project implemented under the Operational Program Research and Development financed by European Fund for Regional Development ITMS 26220120015 (Slovak Republic) and all colleagues from Institute of Biodiversity and Biosafety, Slovak University of Agriculture in Nitra for their help with image analysis. Ananias Pascoal, Georgina Tolentino and Let ıcia Estevinho would like to thank Fundac¸~ao para a Ci^encia e Tecnologia (FCT), Programa Operacional Pontencial Humano (POPH) and European Union (EU) for his Postdoctoral grant SFRH/BPD/91380/2012. Wiebke Sickel, Markus Ankenbrand, Gudrun Grimmer, Frank F€orster, Ingolf Steffan-Dewenter and Alexander Keller thank the financial support by the DFG Collaborative Research Center 1047, Insect Timing. MJA was further supported by a grant of the German Excellence Initiative to the Graduate School of Life Sciences of the University of W€urzburg. They are grateful to the members of the Departments of Animal Ecology and Tropical Biology; Bioinformatics; and Human Genetics, University of W€urzburg, for constructive input on the design of the workflow. Additionally thank to the Department of Human Genetics, especially S. Rost, for granting access to the Illumina MiSeq device. Zivoslav Te si c, Mirjana Mosi c, Aleksandar Kosti c, Mirjana Pe si c, Du sanka Milojkovi c-Opsenica thank the Ministry of Education, Science and Technological Development of Serbia, Grants 172017 and TR 31069. Gina Tolentino would like to thank the Mountain Research Center (CIMO), Agricultural College of Bragança, Polytechnic Institute of Braganc¸a for his research grant in the project titled " Development of new bee products in biological production way.”pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCampos, Maria G.; Anjos, Ofélia; Chica, Manuel; Campoy, Pascual; Nozkova, Janka; Almaraz-Abarca, Norma; Barreto, Lidia M. R. C.; Nordi, João Carlos; Estevinho, Leticia M.; Pascoal, Ananias; Paula, Vanessa Branco; Choupina, Altino; Dias, Luis G.; Tešić, Živoslav L. J.; Mosić, Mirjana D.; Kostić, Aleksandar Ž.; Pešić, Mirjana B.; Milojković-Opsenica, Dušanka M.; Sickel, Wiebke; Ankenbrand, Markus J.; Grimmer, Gudrun; Steffan-Dewenter, Ingolf; Keller, Alexander; Förster, Frank; Tananaki, Chrysoula H.; Liolios, Vasilios; Kanelis, Dimitrios; Rodopoulou, Maria-Anna; Thrasyvoulou, Andreas; Paulo, Luísa; Kast, Christina; Lucchetti, Matteo A.; Glauser, Gaëtan; Lokutova, Olena; Almeida-Muradian, Ligia Bicudo de; Szczęsna, Teresa; Carreck, Norman L. (2021). Standard methods for pollen research. Journal of Apicultural Research. ISSN 0021-8839. 60:4, p. 1-111pt_PT
dc.identifier.doi10.1080/00218839.2021.1948240pt_PT
dc.identifier.issn0021-8839
dc.identifier.urihttp://hdl.handle.net/10198/23994
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCoimbra Chemistry Center
dc.relationForest Research Centre
dc.relationOBTENTION OF NEW PRODUCTS BASED HONEY: MEAD AND SPARKLING MEAD
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectABTSpt_PT
dc.subjectAlkaloidspt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAntimutagenicpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectBee pollenpt_PT
dc.titleStandard methods for pollen researchpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCoimbra Chemistry Center
oaire.awardTitleForest Research Centre
oaire.awardTitleOBTENTION OF NEW PRODUCTS BASED HONEY: MEAD AND SPARKLING MEAD
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00313%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00239%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F91380%2F2012/PT
oaire.citation.endPage109pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Apicultural Researchpt_PT
oaire.citation.volume60pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFARH
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person.familyNamePaula
person.familyNameChoupina
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person.givenNameLetícia M.
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person.givenNameAltino
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