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Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia coli Linked to Sternal Bursitis in Chickens: A One Health Perspective

2025Journal article Open access
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datacite.subject.fosCiências Agrárias::Ciências Veterinárias
datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg02:Erradicar a Fome
dc.contributor.authorRibeiro, Jessica
dc.contributor.authorSilva, Vanessa
dc.contributor.authorFreitas, Catarina
dc.contributor.authorPinto, Pedro
dc.contributor.authorVieira-Pinto, Madalena
dc.contributor.authorBatista, Rita
dc.contributor.authorNunes, Alexandra
dc.contributor.authorGomes, João Paulo
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorBarros, Lillian
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorReis, Filipa S.
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2025-11-14T15:13:15Z
dc.date.available2025-11-14T15:13:15Z
dc.date.issued2025
dc.description.abstractSternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 Escherichia coli isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, including ST155, ST201, and ST58. Resistance to tetracycline and ciprofloxacin was common, and several isolates carried genes encoding beta-lactamases, including blaTEM-1B. Chromosomal mutations associated with quinolone and fosfomycin resistance (e.g., gyrA p.S83L, glpT_E448K) were also identified. WGS revealed a high number of virulence-associated genes per isolate (58-96), notably those linked to adhesion (fim, ecp clusters), secretion systems (T6SS), and iron acquisition (ent, fep, fes), suggesting strong pathogenic potential. Many isolates harbored virulence markers typical of ExPEC/APEC, such as iss, ompT, and traT, even in the absence of multidrug resistance. Our findings suggest that E. coli from sternal bursitis may act as reservoirs of resistance and virulence traits relevant to animal and public health. This highlights the need for including such lesions in genomic surveillance programs and reinforces the importance of integrated One Health approaches.eng
dc.description.sponsorshipThis work was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of projects UI/00772 and LA/P/0059/2020. It also received national financial support through FCT/MCTES (PIDDAC), specifically via CIMO (UIDB/00690/2020 and UIDP/00690/2020) and the SusTEC Associated Laboratory (LA/P/0007/2020). Jessica Ribeiro acknowledges the funding received from FCT through her doctoral fellowship (grant number 2023.00592.BD). The authors also recognize the national support granted by FCT through employment contracts, namely the institutional contract awarded to S. A. Heleno and the individual scientific employment contract of F. S. Reis (reference 2021.03728.CEECIND).
dc.identifier.citationRibeiro, J.; Silva, V.; Freitas, C.; Pinto, P.; Vieira-Pinto, M.; Batista, R.; Nunes, A.; Gomes, J.P.; Pereira, J.E.; Igrejas, G.; et al. Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia coli Linked to Sternal Bursitis in Chickens: A One Health Perspective. Veterinary Sciences. ISSN 2306-7381. 12:7, p. 1-20
dc.identifier.doi10.3390/vetsci12070675
dc.identifier.issn2306-7381
dc.identifier.urihttp://hdl.handle.net/10198/35075
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relation.ispartofVeterinary Sciences
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/
dc.subjectEscherichia coli
dc.subjectBroilers
dc.subjectPoultry
dc.subjectSternal bursitis
dc.subjectAntimicrobial resistance
dc.subjectGenetic lineages
dc.titleGenomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia coli Linked to Sternal Bursitis in Chickens: A One Health Perspectiveeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage20
oaire.citation.issue7
oaire.citation.startPage1
oaire.citation.titleVeterinary Sciences
oaire.citation.volume12
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBarros
person.familyNameHeleno
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person.givenNameLillian
person.givenNameSandrina A.
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person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9159-0530
person.identifier.ridJ-3600-2013
person.identifier.ridL-5951-2014
person.identifier.ridI-2093-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id36982144400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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