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Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility

2025Artigo científico Acesso aberto
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Industrial
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorBorges, Ana Margarida
dc.contributor.authorOrdóñez-Díaz, José Luis
dc.contributor.authorAquino, Yara
dc.contributor.authorMoreno-Rojas, José M.
dc.contributor.authorCalvo, María Luisa Martín
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorCalhelha, Ricardo C.
dc.date.accessioned2026-06-12T11:12:38Z
dc.date.available2026-06-12T11:12:38Z
dc.date.issued2025
dc.description.abstractThe incorporation of bioactive natural compounds into biomedical applications offers a promising route to enhance therapeutic efficacy while supporting sustainability. In this study, we investigated the synergistic potential of Sericin, a silk-derived biopolymer, and Chelidonium majus L. (C. majus), a medicinal plant with a diverse phenolic profile, in relation to biological activities relevant for wound care and infection control. A combined experimental strategy was applied, integrating detailed chemical characterization of C. majus extracts with antimicrobial and cytocompatibility assays across different Sericin–plant extract ratios (1:1, 1:2, 2:2, and 2:1). Phytochemical analysis identified and quantified 57 phenolic compounds, including high levels of flavonoids (quercetin, kaempferol, isorhamnetin) and phenolic acids (caffeic and ferulic acid). Salicylic acid (123.6 µg/g), feruloyltyramine (111.8 µg/g), and pinocembrin (98.4 µg/g) were particularly abundant, compounds previously reported to disrupt microbial membranes and impair bacterial viability. These metabolites correlated with the strong antimicrobial activity of C. majus against Gram-positive strains (MIC = 5–10 mg/mL). In combination with Sericin, antimicrobial performance was ratio-dependent, with higher proportions of C. majus (2:1) retaining partial inhibitory effects. Cytocompatibility assays with HFF1 fibroblasts demonstrated low antiproliferative activity across most formulations (GI50 > 400 µg/mL), supporting their potential safety in topical applications. Collectively, the results indicate a concentration-dependent interaction between C. majus phenolics and the Sericin protein matrix, reinforcing their suitability as candidates for natural-based wound healing materials. Importantly, the valorization of Sericin, an underutilized byproduct of the silk industry, together with a widely accessible medicinal plant, underscores the ecological and economic sustainability of this approach. Overall, this work supports the exploration of the development of biomaterials with potential for advancing tissue repair and wound management.eng
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT. Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). National funding by FCT. P.I. through the scientific employment program contract for the contract of R. Calhelha (CEEC Institutional dOI:10.54499/CEECINST/00016/2018/CP1505/CT0009) and through the Project Wastesilk ( http://doi.org/10.54499/PTDC/BTA-BTA/0696/2020 ). Jos\u00E9 Luis Ord\u00F3\u00F1ez-D\u00EDaz was supported by a postdoctoral contract from Junta de Andaluc\u00EDa within the PAIDI 2020 program (POSTDOC_21_00914) and Jos\u00E9 Castillejo mobility program from the Spanish Ministry of Universities (CAS23/00285). This research was funded by Science and Technology (FCT. Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). National funding by FCT. P.I. through the scientific employment program contract for the contract of R. Calhelha (CEEC Institutional DOI:10.54499/CEECINST/00016/2018/CP1505/CT0009) and through the project Wastesilk (http://doi.org/10.54499/PTDC/BTA-BTA/0696/2020).
dc.identifier.citationBorges, Ana Margarida; Ordóñez-Díaz, José Luis; Aquino, Yara; Moreno-Rojas, José M.; Calvo, María Luisa Martín; Vaz, Josiana A.; Calhelha, Ricardo C. (2025). Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility. International Journal of Molecular Sciences. ISSN 1661-6596. 26:20, p. 1-23
dc.identifier.doi10.3390/ijms26209911
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10198/36866
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSustainable healthcare
dc.subjectBiomaterial
dc.subjectSilk
dc.subjectGreater celandine
dc.subjectPhenolic compounds
dc.subjectHPLC-MS/MS
dc.subjectAntimicrobial
dc.subjectCytocompatibility
dc.titleAnalysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibilityeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberLA/P/0007/2020
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage23
oaire.citation.issue20
oaire.citation.startPage1
oaire.citation.titleInternational Journal of Molecular Sciences
oaire.citation.volume26
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBorges
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person.familyNameCalhelha
person.givenNameAna Margarida
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person.givenNameJosiana A.
person.givenNameRicardo C.
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person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.orcid0009-0009-8809-0541
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0002-6801-4578
person.identifier.ridJ-2172-2014
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id6507978333
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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