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Towards honey authentication: assessing European honey entomological origin by a molecular identification approach

2017Conference object Open access
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dc.contributor.authorSoares, Sónia
dc.contributor.authorGrazina, Liliana
dc.contributor.authorMafra, Isabel
dc.contributor.authorCosta, Joana
dc.contributor.authorPinto, M. Alice
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2018-02-15T11:45:11Z
dc.date.available2018-02-15T11:45:11Z
dc.date.issued2017
dc.description.abstractFollowing the European Union (EU) legislation, honey should be produced by the western honey bee, Apis mellifera. Across Europe, 10 different A. mellifera subspecies can be found, comprising 3 different lineages (A, M and C) based on mtDNA [1]. In general, honey bees occupy allopatric geographical ranges according to their evolutionary lineages, allowing to establish an entomological origin for honey produced in different EU countries. Additionally, several honeys with protected designation of origin (PDO) detail the subspecies traditionally used in their production [2]. While numerous works focused on the botanical and/or geographical authenticity of honey, only a few have attempted its entomological authentication. For that purpose, DNA-based methods have been considered as the most suitable tools since they allow the unequivocal species identification. So far, only few works described the use of DNAbased methods to establish the entomological origin of honey [3,4] and those were focused on different species of honey bees, including Meliponini and/or Trigonini stingless bees. To our knowledge, this is the first attempt to distinguish among different European honey bee subspecies commonly used in honey production, with further application to honey authentication. In this work, DNA markers were developed for the differentiation of A. mellifera subspecies DNA in honey. For this purpose, individuals of A. m. iberiensis lineage A (n=22) from Portugal and Spain (n=5), A. m. iberiensis lineage M from Spain (n=7), A. m. mellifera lineage M from France, Netherlands, Scotland and Norway (n=7), A. m. ligustica lineage C from Italy (n=4), A. m. carnica lineage C from Croacia and Serbia (n=4) and commercial Buckfast lineage C bees (n=10) were tested. Different sets of primers were designed targeting the cytochrome oxidase I gene. The specificity and sensitivity of the designed primers were assayed by qualitative polymerase chain reaction (PCR). Species-specific primers successfully allowed the identification of A. m. iberiensis lineage A by end-point PCR. The use of real-time PCR coupled with High Resolution Melting analysis allowed the separation of A. mellifera honey bee subspecies in different clusters according to their lineages. The developed methodologies were applied to the analysis of authentic honey samples from Portugal (produced by A. m. iberiensis lineage A), Spain (produced by A. m. iberiensis lineage M), and Italy (produced by A. m. ligustica lineage C), allowing its successful entomological origin identification.pt_PT
dc.description.sponsorshipThis work has been supported by FCT (Fundação para a Ciência e Tecnologia) through project UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/ MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by the project NORTE-01-0145-FEDER-000011. S. Soares and J. Costa are grateful to FCT grants (SFRH/ BPD/102404/2014 and SFRH/BD/75091/2010) financed by POPH-QREN (subsidized by FSE and MCTES).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSoares, Sónia; Grazina, Liliana; Mafra, Isabel; Costa, Joana; Pinto, M. Alice; Oliveira, M.B.P.P., Amaral, J.S. (2017). Towards honey authentication: assessing European honey entomological origin by a molecular identification approach. In Food Integrity Conference 2017. Parma. p. 186-187. ISBN 978-8894106657pt_PT
dc.identifier.isbn978-8894106657
dc.identifier.urihttp://hdl.handle.net/10198/15764
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationSFRH/ BPD/102404/2014pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoneypt_PT
dc.subjectApis mellifera subspeciespt_PT
dc.subjectAuthenticitypt_PT
dc.subjectWestern honey beept_PT
dc.titleTowards honey authentication: assessing European honey entomological origin by a molecular identification approachpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F75091%2F2010/PT
oaire.citation.conferencePlaceParma, Italypt_PT
oaire.citation.titleFood Integrity Conference 2017pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameAmaral
person.givenNameM. Alice
person.givenNameJoana S.
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery0667fe04-7078-483d-9198-56d167b19bc5
relation.isProjectOfPublication40f15c30-b6e8-4474-ae1d-d7256c7af84e
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