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Flow of red blood cells in microchannel networks: in vitro studies

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dc.contributor.authorCidre, Diana
dc.contributor.authorRodrigues, Raquel Oliveira
dc.contributor.authorFaustino, Vera
dc.contributor.authorPinto, Elmano
dc.contributor.authorPinho, Diana
dc.contributor.authorBento, David
dc.contributor.authorFernandes, Carla S.
dc.contributor.authorDias, Ricardo P.
dc.contributor.authorLima, Rui A.
dc.date.accessioned2014-08-07T13:16:21Z
dc.date.available2014-08-07T13:16:21Z
dc.date.issued2013
dc.description.abstractBlood exhibits unique flow characteristics on micro-scale level, due to the complex biochemical structure of Red Blood Cells (RBCs) and their response to both shear and extensional flow, which influence the rheological properties and flow behavior of blood [1,2]. In the past years, several in vitro studies where made and have revealed some physiologically significant phenomena, such as Fahraeus and Fahraeus-Lindqvist effect, that played a key role in recent developments of lab-onchip devices for blood sampling, analysis and cell culturing. However, the blood flow in microvascular networks phenomena it remains incompletely understood. Thus, it is important to investigate in detail the behavior of RBCs flow occurring at a microchannel network, such as with divergent and convergent bifurcations. Previews in vitro studies in microchannels with a simple divergent and convergent bifurcation, have shown a pronounced cell depleted zone immediately downstream of the apex of the convergent bifurcation. In the present work, by using a highspeed video microscopy system, we investigated the cell depleted zone in a microchannel network. The working fluid used in this study was dextran 40 (Dx40) containing about 10% of haematocrit level (10 Hct) of ovine red blood cells. The high-speed video microscopy system used in our experiments consists of an inverted microscope (IX71, Olympus, Japan) combined with a highspeed camera (i-SPEED LT, Olympus). A syringe pump Apparatus (PHD ULTRATM) with 1 ml syringe (Terumo) was used to push the working fluids through the microchannel network. Additionally, we investigated the effect of the flow rate on the formation of the cell free layer.por
dc.description.sponsorshipThe authors acknowledge the financial support provided by: PTDC/SAU-BEB/108728/2008, PTDC/SAU-BEB/105650/2008, PTDC/EME-MFE/099109/2008 and PTDC/SAU-ENB/116929/2010 from FCT (Science and Technology Foundation), COMPETE, QREN and European Union (FEDER).
dc.identifier.citationCidre, Diana; Rodrigues, Raquel O.; Faustino, Vera; Pinto, Elmano; Pinho, Diana; Bento, David; Fernandes, Carla S.; Dias, Ricardo P.; Lima, R. (2013). Flow of red blood cells in microchannel networks: in vitro studies. In Iberian Meeting on Rheology. Málagapor
dc.identifier.urihttp://hdl.handle.net/10198/10039
dc.language.isoengpor
dc.relationHemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks.
dc.relationANEURYSM - Hemodynamic Study in Cerebral Aneurysms
dc.relationBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
dc.subjectMicrochannel networkspor
dc.subjectCell-free layerpor
dc.titleFlow of red blood cells in microchannel networks: in vitro studiespor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleHemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks.
oaire.awardTitleANEURYSM - Hemodynamic Study in Cerebral Aneurysms
oaire.awardTitleBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F108728%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEME-MFE%2F099109%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT
oaire.citation.conferencePlaceMálagapor
oaire.citation.titleIberian Meeting on Rheologypor
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
person.familyNamePinho
person.familyNameFernandes
person.familyNameDias
person.familyNameLima
person.givenNameDiana
person.givenNameCarla S.
person.givenNameRicardo P.
person.givenNameRui A.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-idF319-3D67-9DB7
person.identifier.ciencia-idEE12-C3FB-349D
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0002-3138-7493
person.identifier.orcid0000-0003-1503-998X
person.identifier.orcid0000-0003-3428-637X
person.identifier.ridA-4269-2015
person.identifier.ridH-5157-2016
person.identifier.scopus-author-id8253740700
person.identifier.scopus-author-id7102885151
person.identifier.scopus-author-id18437397800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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