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A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods

2016Journal article Open access
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dc.contributor.authorRejili, M.
dc.contributor.authorFernandes, Telmo J.R.
dc.contributor.authorDinis, Ana Maria
dc.contributor.authorPereira, J.A.
dc.contributor.authorBaptista, Paula
dc.contributor.authorSantos, Sónia A.P.
dc.contributor.authorLino-Neto, Teresa
dc.date.accessioned2016-11-15T15:36:48Z
dc.date.available2016-11-15T15:36:48Z
dc.date.issued2016
dc.description.abstractBactrocera oleae (Rossi) (Diptera: Tephritidae) is considered the most devastating pest of the olive tree worldwide. In an effort to develop management and biological control strategies against this pest, new molecular tools are urgently needed. In this study, we present the design of B. oleae-specific primers based on mitochondrial DNA sequences of cytochrome oxidase subunit I (COI) gene. Two pairs of B. oleae-specific primers were successfully designed and named as SBo1-F/SBo1-R and SBo2-F/SBo1-R, being able to amplify 108 and 214 bp COI fragments, respectively. The specificity of designed primers was tested by amplifying DNA from phylogenetically related (i.e. Diptera order) and other non-pest insects living in olive groves from the Mediterranean region. When using these primers on a PCR-based diagnostic assay, B. oleae DNA was detected in the gut content of a soil-living insect, Pterostichus globosus (Fabricius) (Coleoptera: Carabidae). The detection of B. oleae DNA in the guts of arthropods was further optimized by adding bovine serum albumin enhancer to the PCR reaction, in order to get a fast, reproducible and sensitive tool for detecting B. oleae remains in the guts of soil-living arthropods. This molecular tool could be useful for understanding pest-predator relationships and establishing future biological control strategies for this pest.pt_PT
dc.identifier.citationRejili, M.; Fernandes, T.; Dinis, Ana Maria de Sousa Pereira; Pereira, J.A.; Baptista, Paula; Santos, Sónia A.P.; Lino-Neto, Teresa (2016). A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods. Bulletin of Entomological Research. ISSN 0007-4853. 106:5, p. 695-699pt_PT
dc.identifier.doi10.1017/S000748531600050Xpt_PT
dc.identifier.issn0007-4853
dc.identifier.urihttp://hdl.handle.net/10198/13494
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCambridge University Presspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBactrocera oleaept_PT
dc.subjectSpecific primerspt_PT
dc.subjectDiagnostic PCRpt_PT
dc.subjectFeeding assaypt_PT
dc.titleA PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/EXCL%2FAGR-PRO%2F0591%2F2012/PT
oaire.citation.endPage699pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage695pt_PT
oaire.citation.titleBulletin of Entomological Researchpt_PT
oaire.citation.volume106pt_PT
oaire.fundingStream5876-PPCDTI
person.familyNamePereira
person.familyNameBaptista
person.familyNameSantos
person.givenNameJose Alberto
person.givenNamePaula
person.givenNameSónia
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.ciencia-id5C1D-7CF5-601C
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0001-6331-3731
person.identifier.orcid0000-0003-1500-6360
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id14051688000
person.identifier.scopus-author-id7102756340
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublicatione662327b-d6c2-4db8-8e5d-60889d2a71c6
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9
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