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Comparative methods to evaluate the antioxidant capacity of propolis: an attempt to explain the differences

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dc.contributor.authorPaula, Vanessa B.
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorCardoso, Susana M.
dc.contributor.authorDias, L.G.
dc.date.accessioned2023-02-01T11:10:50Z
dc.date.available2023-02-01T11:10:50Z
dc.date.issued2023
dc.description.abstractPropolis is a natural product produced by bees that contains a complex mixture of compounds, including phenolic compounds and flavonoids. These compounds contribute to its biological activities, such as antioxidant capacity. This study analysed the pollen profile, total phenolic content (TPC), antioxidant properties, and phenolic compound profile of four propolis samples from Portugal. The total phenolic compounds in the samples were determined by six different techniques: four different Folin-Ciocalteu (F-C) methods, spectrophotometry (SPECT), and voltammetry (SWV). Of the six methods, SPECT allowed the highest quantification, while SWV achieved the lowest. The mean TPC values for these methods were 422 & PLUSMN; 98 and 47 & PLUSMN; 11 mg GAE/g sample, respectively. Antioxidant capacity was determined by four different methods: DPPH, FRAP, original ferrocyanide (OFec), and modified ferrocyanide (MFec). The MFec method gave the highest antioxidant capacity for all samples, followed by the DPPH method. The study also investigated the correlation between TPC and antioxidant capacity with the presence of hydroxybenzoic acid (HBA), hydroxycinnamic acid (HCA), and flavonoids (FLAV) in propolis samples. The results showed that the concentrations of specific compounds in propolis samples can significantly impact their antioxidant capacity and TPC quantification. Analysis of the profile of phenolic compounds by the UHPLC-DAD-ESI-MS technique identified chrysin, caffeic acid isoprenyl ester, pinocembrin, galangin, pinobanksin-3-O-acetate, and caffeic acid phenyl ester as the major compounds in the four propolis samples. In conclusion, this study shows the importance of the choice of method for determining TPC and antioxidant activity in samples and the contribution of HBA and HCA content to their quantification.pt_PT
dc.description.sponsorshipThe authors are grateful to: Nelson De Moura of the company Ecoapis Unipessoal Lda (http://www.ecoapis.pt (accessed on 15 March 2023)) for supplying the propolis samples; Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPaula, Vanessa B.; Estevinho, Leticia M.; Cardoso, Susana M.; Dias, L.G. (2023). Comparative methods to evaluate the antioxidant capacity of propolis: an attempt to explain the differences. Molecules. eISSN 1420-3049. 28:12, p. 1-23pt_PT
dc.identifier.doi10.3390/molecules28124847pt_PT
dc.identifier.eissn1420-3049
dc.identifier.urihttp://hdl.handle.net/10198/26721
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPropolispt_PT
dc.subjectTotal phenolic compoundspt_PT
dc.subjectAntioxidant capacitypt_PT
dc.subjectUHPLC-DAD-ESI-MSpt_PT
dc.titleComparative methods to evaluate the antioxidant capacity of propolis: an attempt to explain the differencespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMoleculespt_PT
person.familyNamePaula
person.familyNameEstevinho
person.familyNameDias
person.givenNameVanessa B.
person.givenNameLetícia M.
person.givenNameLuís G.
person.identifier.ciencia-id5F16-A866-562D
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.orcid0000-0001-7281-8598
person.identifier.orcid0000-0002-9249-1948
person.identifier.orcid0000-0002-1210-4259
person.identifier.scopus-author-id6506577664
person.identifier.scopus-author-id23569169900
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication960f5ea5-510b-491e-b931-6ab78ab72054
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublicationeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isAuthorOfPublication.latestForDiscoveryeac8c166-4056-4ed0-8d8d-7ecb2c4481a5

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