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Advisor(s)
Abstract(s)
A espécie E. globulus é considerada a principal fonte mundial de óleo de eucalipto,
sendo este constituído por uma ampla gama de compostos, sendo o mais importante
o monoterpeno 1,8-cineol. O óleo de eucalipto é amplamente utilizado para fins medicinais,
tais como alívio da tosse, dor de garganta e outras infeções, como antissético e
utilizado como ingrediente em produtos farmacêuticos e cosméticos. O objetivo deste
trabalho foi avaliar a atividade antimicrobiana de óleos essenciais do fruto e folhas
do eucalipto obtidos por hidrodestilação em aparelho de Clevenger e também de dois
óleos comerciais. No estudo usaram-se bactérias Gram-positivas (Staphylococcus aureus,
Bacillus cereus, Bacillus subtilis, Enterococcus faecalis), Gram-negativas (Escherichia
coli, Klebsiella pneumoniae, Enterobacter sp., Proteus mirabilis, Pseudomonas aeruginosa)
e o fungo Candida albicans. A atividade antimicrobiana dos óleos essenciais do fruto
e folhas do eucalipto foi avaliada pelo método da macrodiluição em caldo, tendo sido
testadas diferentes concentrações (2,5% a 0,04%, v/v) para determinação da concentração
mínima inibitória (CMI). O estudo realizado mostrou que os óleos essenciais
comerciais (n=2) e extraídos das folhas (n=3), apresentaram atividade inibitória no crescimento
de todos os microrganismos estudados à exceção de P. aeruginosa, cujo crescimento
não foi suscetível à presença dos óleos essenciais, mesmo na concentração
mais elevada (2,5% v/v). De uma forma geral, o óleo essencial do fruto apresentou uma
eficácia inferior comparativamente às amostras comerciais e das folhas, à exceção da
bactéria S. aureus, para a qual a amostra do óleo de fruto apresentou o mais baixo valor
de CMI (0,16%, v/v).
E. globulus species is considered the main worldwide source of eucalyptus oil, which consists of a wide range of compounds, being monoterpene 1,8-cineol the most important one. Eucalyptus oil is widely used for medicinal purposes such as cough relief, sore throat and other infections, and also as an antiseptic and as a component in pharmaceuticals and cosmetics. The objective of this work was to evaluate the antimicrobial activity of essential oils of the eucalyptus fruit and leaves obtained by hydrodistillation in a Clevenger apparatus. Additionally, two commercial oils were also studied. Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Enterococcus faecalis), Gram-negative (Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Proteus mirabilis, Pseudomonas aeruginosa) and the fungus Candida albicans were used in the study. The antimicrobial activity of the assayed essential oils was evaluated by the broth macrodilution method. Different concentrations (2.5% to 0.04%, v/v) were tested for minimum inhibitory concentration (MIC) determination. The study showed that commercial essential oils (n=2) and those extracted from the leaves (n=3) showed inhibitory activity against all the studied microorganisms, with the exception of P. aeruginosa, which was not inhibited even by the highest concentration of the essential oil (2.5% v/v). In general, the essential oil of the fruit was less effective compared to commercial and leaf samples, with the exception of S. aureus, for which this oil presented the lowest MIC value (0.16 %, v/v).
E. globulus species is considered the main worldwide source of eucalyptus oil, which consists of a wide range of compounds, being monoterpene 1,8-cineol the most important one. Eucalyptus oil is widely used for medicinal purposes such as cough relief, sore throat and other infections, and also as an antiseptic and as a component in pharmaceuticals and cosmetics. The objective of this work was to evaluate the antimicrobial activity of essential oils of the eucalyptus fruit and leaves obtained by hydrodistillation in a Clevenger apparatus. Additionally, two commercial oils were also studied. Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Enterococcus faecalis), Gram-negative (Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Proteus mirabilis, Pseudomonas aeruginosa) and the fungus Candida albicans were used in the study. The antimicrobial activity of the assayed essential oils was evaluated by the broth macrodilution method. Different concentrations (2.5% to 0.04%, v/v) were tested for minimum inhibitory concentration (MIC) determination. The study showed that commercial essential oils (n=2) and those extracted from the leaves (n=3) showed inhibitory activity against all the studied microorganisms, with the exception of P. aeruginosa, which was not inhibited even by the highest concentration of the essential oil (2.5% v/v). In general, the essential oil of the fruit was less effective compared to commercial and leaf samples, with the exception of S. aureus, for which this oil presented the lowest MIC value (0.16 %, v/v).
Description
Keywords
Atividade antimicrobiana Óleos essenciais Eucalipto
Citation
Cidres, Eliana Soraia Santos Alves; Queiroz, A.M.; Amaral, J.S. (2017). Avaliação da atividade antimicrobiana de óleos essenciais de eucalipto (Eucalyptus globulus) = Evaluation of the antimicrobial activity of essential oils of eucalyptus (Eucalyptus globulus). In V Encontro de Jovens Investigadores do Instituto Politécnico de Bragança: livro de resumos. Bragança: Instituto Politécnico. ISBN 978-972-745-235-4
Publisher
Instituto Politécnico de Bragança