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Chemical Characterization and Differential Lipid-Modulating Effects of Selected Plant Extracts from Côa Valley (Portugal) in a Cell Model for Liver Steatosis

2025Journal article Open access
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datacite.subject.fosCiências Agrárias::Agricultura, Silvicultura e Pescas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorAmorim, Ricardo
dc.contributor.authorMarques, Mário Pedro
dc.contributor.authorMelim, Catarina
dc.contributor.authorVarela, Carla
dc.contributor.authorSardão, Vilma A.
dc.contributor.authorTeixeira, José
dc.contributor.authorDias, Maria Inês
dc.contributor.authorBarros, Lillian
dc.contributor.authorOliveira, Paulo J.
dc.contributor.authorCabral, Célia
dc.date.accessioned2025-04-07T15:29:51Z
dc.date.available2025-04-07T15:29:51Z
dc.date.issued2025
dc.description.abstractCôa Valley, located in the northeast of Portugal, harbors more than 500 medicinal plant species. Among them, four species stand out due to their traditional uses: Equisetum ramosissimum Desf. (hemorrhages, urethritis, hepatitis), Rumex scutatus L. subsp. induratus (Boiss. and Reut.) Malag. (inflammation, constipation), Geranium purpureum Vill., and Geranium lucidum L. (pain relief, gastric issues). Given their rich ethnomedicinal history, we evaluated their protective effects on an in vitro model of metabolic dysfunction-associated steatotic liver disease (MASLD). Methods: Decoction (D) and hydroalcoholic (EtOH80%) extracts were prepared and chemically characterized. Their safety profile and effects on lipid accumulation were assessed in palmitic acid (PA)-treated HepG2 cells using resazurin, sulforhodamine B, and Nile Red assays. Results: Chemical analysis revealed diverse phenolic compounds, particularly kaempferol derivatives in E. ramosissimum. All extracts showed minimal cytotoxicity at 25–50 µg/mL. At 100 µg/mL, only E. ramosissimum extracts maintained high cell viability. In the lipotoxicity model, E. ramosissimum decoction demonstrated the most potent effect, significantly reducing PA-induced neutral lipid accumulation in a dose-dependent manner, while other extracts showed varying degrees of activity. Conclusions: These findings highlight E. ramosissimum’s decoction, rich in kaempferol derivatives, as particularly effective in reducing lipid accumulation in this MASLD cell model while also providing a comprehensive characterization of traditionally used plants from the Côa Valley region.eng
dc.description.sponsorshipThis work was funded by the Foundation for Science and Technology (FCT, Por- tugal) through the following projects: UIDB/04539/2020—DOI: 10.54499/UIDB/04539/2020, UIDP/04539/2020—DOI: 10.54499/UIDP/04539/2020, LA/P/0058/2020—DOI: 10.54499/UIDP/ 04539/2020, CôaMedPlants project: COA/BRB/0019/2019—DOI: 10.54499/COA/BRB/0019/2019, and through national funds FCT/MCTES to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020) and M.I.D and L.B contracts (CEEC Institutional)
dc.identifier.citationAmorim, Ricardo; Marques, Mário Pedro; Melim, Catarina; Varela, Carla; Sardão, Vilma A.; Teixeira, José; Dias, Maria Inês; Barros, Lillian; Oliveira, Paulo J.; Cabral, Célia (2025). Chemical Characterization and Differential Lipid-Modulating Effects of Selected Plant Extracts from Côa Valley (Portugal) in a Cell Model for Liver Steatosis. Pharmaceuticals. ISSN 1424-8247. 18:1, p. 1-20
dc.identifier.doi10.3390/ph18010039
dc.identifier.issn1424-8247
dc.identifier.urihttp://hdl.handle.net/10198/34403
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relationCOA/BRB/0019/2019
dc.relation.ispartofPharmaceuticals
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCôa Valley (Portugal)
dc.subjectPlant extracts
dc.subjectEquisetum ramosissimum Desf.
dc.subjectMASLD
dc.subjectLipid-lowering effect
dc.titleChemical Characterization and Differential Lipid-Modulating Effects of Selected Plant Extracts from Côa Valley (Portugal) in a Cell Model for Liver Steatosiseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage20
oaire.citation.issue1
oaire.citation.startPage1
oaire.citation.titlePharmaceuticals
oaire.citation.volume18
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDias
person.familyNameBarros
person.givenNameMaria Inês
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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