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Towards authentication of Korean ginseng-containing foods: differentiation of five Panax species by a novel diagnostic tool

2021Journal article Open access
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dc.contributor.authorGrazina, Liliana
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorCosta, Joana
dc.contributor.authorMafra, Isabel
dc.date.accessioned2021-08-05T14:00:25Z
dc.date.available2021-08-05T14:00:25Z
dc.date.issued2021
dc.description.abstractPanax ginseng C.A. Meyer (Korean ginseng) is one of the most valuable medicinal plants, recognised for its neuroprotection and other beneficial health effects. It is present in a wide range of food products, namely plant food supplements (PFS) and herbal infusions. However, other Panax species, having distinct therapeutic effects, are also known as ginseng, pointing out the need of authenticating such products. The present work aims at proposing a new high-resolution melting (HRM) method to differentiate five Panax species (P. ginseng, P. quinquefolius, P. notoginseng, P. japonicus and P. trifolius), targeting the gene encoding the dammarenediol synthase, involved in the biosynthesis pathway of ginsenosides. A Panax-specific real-time PCR assay was successfully developed with high analytical performance parameters (Efficiency = 100.5 %, R2 = 0.995, dynamic range 10 ng-1 pg of ginseng DNA). Panax DNA was detected in 17 out of 23 ginseng-containing commercial foods, including herbal infusions and PFS. For the first time, HRM analysis differentiated five Panax species with high level of confidence (>98 %), which corroborated sequencing data. Fourteen products were successfully clustered, being all except one in accordance with their labelling statements. Therefore, the present work proposes a reliable and high-throughput tool to authenticate ginseng products that could be useful for control laboratories.pt_PT
dc.description.sponsorshipThe authors acknowledge the support of project UIDB 00690/2020 funded by FCT/MCTES through national funds. The authors are grateful for the supply of leaves from the Botanical Garden of Edinburgh (Edinburgh, Scotland), Botanical Garden of University of Porto (Porto, Portugal), Botanical garden of UTAD (Vila Real, Portugal), as well as to the voucher seeds from the RBG (Kew, Ardingly, West Sussex, UK), USDA-ARS Germplasm (Beltsville, MD, USA) and NCRPIS/Iowa State university (Ames, IA, USA).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrazina, Liliana; Amaral, Joana S.; Costa, Joana; Mafra, Isabel (2021). Towards authentication of Korean ginseng-containing foods: differentiation of five Panax species by a novel diagnostic tool. LWT - Food Science and Technology. ISSN 0023-6438. 151, p. 1-8pt_PT
dc.identifier.doi10.1016/j.lwt.2021.112211pt_PT
dc.identifier.issn0023-6438
dc.identifier.urihttp://hdl.handle.net/10198/23803
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHRM analysispt_PT
dc.subjectHerbal productspt_PT
dc.subjectPlant food supplementspt_PT
dc.subjectAuthenticitypt_PT
dc.titleTowards authentication of Korean ginseng-containing foods: differentiation of five Panax species by a novel diagnostic toolpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleLWT - Food Science and Technologypt_PT
oaire.citation.volume151pt_PT
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd

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