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Development and characterization of high-absorption microencapsulated organic propolis EPP-AF® extract (i-CAPs)

2023Journal article Open access
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dc.contributor.authorBerretta, Andresa Aparecida
dc.contributor.authorLima, Jéssica A. de
dc.contributor.authorFalcão, Soraia
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorAmorim, Nathaly Alcazar
dc.contributor.authorGonçalves, Isabella Salgado
dc.contributor.authorZamarrenho, Luana Gonçalves
dc.contributor.authorBarud, Hernane da Silva
dc.contributor.authorBastos, Jairo Kenupp
dc.contributor.authorJong, David
dc.contributor.authorVilas-Boas, Miguel
dc.date.accessioned2023-12-18T09:56:56Z
dc.date.available2023-12-18T09:56:56Z
dc.date.issued2023
dc.description.abstractThe demand for organic and functional food continues to increase yearly. Among the available functional foods, propolis is a bee product that has various beneficial properties, including antimicrobial, antioxidant, and anti-inflammatory activities. However, it generally is only available in ethanol solution, which has poor bioavailability, as it is relatively insoluble in water. The use of such ethanol extracts is often objectionable because of the alcohol content and because they have a strong and striking taste. Development of alternatives that can efficiently and safely increase solubility in water, and that meet organic production specifications, has been a challenge. To address these concerns, microcapsules were developed using spray-dryer technology from an emulsion based on EPP-AF® propolis and gum arabic (i-CAPS). These propolis-loaded microcapsules were characterized using FT-IR, SEM, TGA, HPLC, and spectrophotometric techniques, along with determination of antimicrobial, antioxidant, antitumor, anti-inflammatory, and antihypercholesterolemic activities, as well as permeability in in vitro models. The production system resulted in microcapsules with a spherical shape and an encapsulation efficiency of 93.7 0.7%. They had IC50s of 2.654 0.062 and 7.342 0.058 g/mL by FRAP and DPPH antioxidant methods, respectively. The EPP-AF® i-CAPS also had superior antimicrobial activity against Gram-positive bacteria. Antitumor activity was calculated based on the concentration that inhibited 50% of growth of AGS, Caco-2, and MCF-7 cell strains, giving results of 154.0 1.0, 117 1.0, and 271.0 25 g/mL, respectively. The microcapsule presentation reduced the permeation of cholesterol by 53.7%, demonstrating antihypercholesterolemic activity, and it improved the permeability of p-coumaric acid and artepillin C. The IC50 for NO production in RAW264.7 cells was 59.0 0.1 g/mL. These findings demonstrate the potential of this new propolis product as a food and pharmaceutical ingredient, though additional studies are recommended to validate the safety of proposed dosages.pt_PT
dc.description.sponsorshipCNPq financially supported this research through the CNPQ/MCTI/SEMPI No. 021/2021 process on RHAE modality, contract number 424727/2021-8. It was also supported by São Paulo Research Foundation (FAPESP) grant #2017/04138-8, CNPq (grant 309614/2021-0; INCT-INFO), CNPq grants 350088/2022-5 and 350089/2022-1, São Paulo Research Foundation (FAPESP) (grant no. 2013/07276-1). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). Thanks to the project GreenHealth, Norte-01- 0145-FEDER-000042.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBerretta, Andresa Aparecida; Lima, Jéssica A. de; Falcão, Soraia; Calhelha, Ricardo C.; Amorim, Nathaly Alcazar; Gonçalves, Isabella Salgado; Zamarrenho, Luana Gonçalves; Barud, Hernane da Silva; Bastos, Jairo Kenupp; Jong, David; Vilas-Boas, Miguel (2023). Development and characterization of high-absorption microencapsulated organic propolis EPP-AF® extract (i-CAPs). Molecules. ISSN 1420-3049. 28:20, p. 1-25pt_PT
dc.identifier.doi10.3390/molecules28207128pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10198/28964
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicroencapsulated propolis extractpt_PT
dc.subjectAntitumorpt_PT
dc.subjectAnti-inflammatorypt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectBioavailabilitypt_PT
dc.subjectPermeabilitypt_PT
dc.titleDevelopment and characterization of high-absorption microencapsulated organic propolis EPP-AF® extract (i-CAPs)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.endPage25pt_PT
oaire.citation.issue20pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume28pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFalcão
person.familyNameCalhelha
person.familyNameVilas-Boas
person.givenNameSoraia
person.givenNameRicardo C.
person.givenNameMiguel
person.identifier711432
person.identifier1693134
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-8665-5280
person.identifier.ridJ-2172-2014
person.identifier.ridI-5949-2013
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id6602648497
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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