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Molecular detection of Philaenus spumarius DNA in predators gut

2017Conference object Open access
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dc.contributor.authorLantero, Ester
dc.contributor.authorVilla, Maria
dc.contributor.authorBenhadi-Marín, Jacinto
dc.contributor.authorPascual, Susana
dc.contributor.authorMatallanas, Beatriz
dc.contributor.authorCallejas, Carmen
dc.contributor.authorBaptista, Paula
dc.contributor.authorPereira, J.A.
dc.date.accessioned2020-12-02T14:51:33Z
dc.date.available2020-12-02T14:51:33Z
dc.date.issued2017
dc.description.abstractX. fastidiosa is one of the most destructive pathogens of olive orchards. Its recent arrival to Europe can cause important losses, with possible drastic consequences for the sector. Philaenus spumarius (Linnaeus 1758) was identified as vector of X. fastidiosa in Europe and finding candidate natural enemies of P. spumarius is essential to design environmentally friendly control strategies against X. fastidiosa. Generalist predators, such as spiders, arise as important potential biocontrol agents. In this context, a set of species-specific primers for detecting the presence of P. spumarius DNA in the gut of predatory arthropods is being developed. All primers will be tested for checking cross-reactive amplification of arthropods DNA and evaluated in heterospecific mixes of nucleic acids. Subsequent feeding trials will be conducted using Synemaglobosum (Fabricius 1775), an important spider species in olive groves from Mirandela, Portugal. These trials will allow determining the detection efficiency of the primers designed and also they will be the starting point to detect predation in field trials. These primers therefore provide a very useful tool for screening the gut contents of potential predators of P. spumarius, and can thus reveal candidate species for this species biological control.pt_PT
dc.description.sponsorshipThis work has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N. 727987 “Xylella fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy XF-ACTORS”.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLantero, Ester; Villa, Maria; Benhadi-Marín, Jacinto; Pascual, Susana; Matallanas, Beatriz; Callejas, Carmen; Baptista, Paula; Pereira, J.A. (2017). Molecular detection of Philaenus spumarius DNA in predators gut. In European conference on Xylella. Palma de Mallorcapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/22908
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationXylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectPhilaenus spumariuspt_PT
dc.titleMolecular detection of Philaenus spumarius DNA in predators gutpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleXylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/727987/EU
oaire.citation.conferencePlacePalma de Mallorca, Españapt_PT
oaire.citation.startPage60pt_PT
oaire.citation.titleEuropean conference on Xylella. Finding answers to a global problem. Finding answers to a global problempt_PT
oaire.fundingStreamH2020
person.familyNameVilla Serrano
person.familyNameBenhadi-Marín
person.familyNameBaptista
person.familyNamePereira
person.givenNameAna María
person.givenNameJacinto
person.givenNamePaula
person.givenNameJose Alberto
person.identifier.ciencia-idB81D-89AC-6368
person.identifier.ciencia-id8D12-5415-20E0
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0001-8353-5438
person.identifier.orcid0000-0002-9804-4145
person.identifier.orcid0000-0001-6331-3731
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57158670600
person.identifier.scopus-author-id14051688000
person.identifier.scopus-author-id57204366348
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscoveryf0354077-bebe-437c-8625-751953199417
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