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Standard methods for molecular research in Apis mellifera

2013Journal article Open access
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dc.contributor.authorEvans, Jay D.
dc.contributor.authorSchwarz, Ryan S.
dc.contributor.authorChen, Yan Ping
dc.contributor.authorBudge, Giles
dc.contributor.authorCornman, Robert S.
dc.contributor.authorDe la Rúa, Pilar
dc.contributor.authorMiranda, Joachim
dc.contributor.authorForet, Sylvain
dc.contributor.authorFoster, Leonard
dc.contributor.authorGauthier, Laurent
dc.contributor.authorGenersch, Elke
dc.contributor.authorGisder, Sebastian
dc.contributor.authorJarosch, Antje
dc.contributor.authorKucharski, Robert
dc.contributor.authorLopez, Dawn
dc.contributor.authorLun, Cheng Man
dc.contributor.authorMoritz, Robin F.A.
dc.contributor.authorMaleszka, Ryszard
dc.contributor.authorMuñoz, Irene
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2013-10-28T09:22:10Z
dc.date.available2013-10-28T09:22:10Z
dc.date.issued2013
dc.description.abstractFrom studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.por
dc.description.sponsorshipFundação para a Ciência e Tecnologiapor
dc.identifier.citationEvans, J.D.; Schwarz, R.; Chen, Y.P.; Budge, G.; Cornman, R. S.; De la Rúa, P.; Miranda, J.R.; Foret, S.; Foster, L.; Gauthier, L.; Genersch, E.; Gisder, S.; Jarosch, A.; Kucharski, R.; Lopez, D.; Lun, C.M.; Moritz, R.F.A.; Maleszka, R.; Muñoz, I.; Pinto, M. Alice (2013). Standard methods for molecular research in Apis melífera. Journal of Apicultural Research. ISSN 0021-8839. 52:4, p. 1-53por
dc.identifier.doi10.3896/IBRA.1.52.4.11
dc.identifier.issn0021-8839
dc.identifier.urihttp://hdl.handle.net/10198/8920
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInternational Bee Research Associationpor
dc.subjectApis melliferapor
dc.subjectIdentificationpor
dc.titleStandard methods for molecular research in Apis melliferapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUnited Kingdompor
oaire.citation.titleJournal of Apicultural Researchpor
oaire.citation.volume52 (4)por
person.familyNamePinto
person.givenNameM. Alice
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id8085507800
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication.latestForDiscovery0667fe04-7078-483d-9198-56d167b19bc5

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