Repository logo
 
Publication

Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells

2020Journal article Open access
Simple item page
dc.contributor.authorStojković, Dejan
dc.contributor.authorDias, Maria Inês
dc.contributor.authorDrakulić, Danijela
dc.contributor.authorBarros, Lillian
dc.contributor.authorStevanović, Milena
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSoković, Marina
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-02-27T17:37:54Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-02-27T17:37:54Z
dc.date.issued2020
dc.description.abstractOnonis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.en_EN
dc.description.sponsorshipThis work has been supported by Ministry of Education, Science and Technological Development of Republic of Serbia (451-03-68/2020-14/200007 and 451-03-68/2020-14/200042). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scientific employment program-contract for M.I. Dias and L. Barros; to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationStojković, Dejan; Dias, Maria Inês; Drakulić, Danijela; Barros, Lillian; Stevanović, Milena; Ferreira, Isabel C.F.R.; Soković, Marina (2020). Methanolic extract of the herb ononis spinosa L. Is an antifungal agent with no cytotoxicity to primary human cells. Pharmaceuticals. ISSN 1424-8247. 13:4, p. 1-13en_EN
dc.identifier.doi10.3390/ph13040078en_EN
dc.identifier.urihttp://hdl.handle.net/10198/23417
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAntibiofilmen_EN
dc.subjectCytotoxicityen_EN
dc.subjectO. spinosaen_EN
dc.titleMethanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cellsen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameDias
person.familyNameBarros
person.familyNameFerreira
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
79.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Plain Text
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus