Repository logo
 
Publication

Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC-UV and ESI- MS combined method

2013Journal article Open access
Simple item page
dc.contributor.authorPereira, Olívia R.
dc.contributor.authorPeres, António M.
dc.contributor.authorSilva, Artur
dc.contributor.authorDomingues, Maria R.M.
dc.contributor.authorCardoso, Susana M.
dc.date.accessioned2014-05-13T09:05:56Z
dc.date.available2014-05-13T09:05:56Z
dc.date.issued2013
dc.description.abstractThymus x citriodorus is a Lamiaceae plant extensively cultivated in Mediterranean region and used for centuries in culinary and in traditional medicine. The present work describes the detailed phenolic composition of T. x citriodorus for the first time, the by means of HPLC-DAD, ESI-MS and MSn and nuclear magnetic resonance (NMR) analyses. The ethanolic extract of T. x citriodorus was analyzed by reversed phase HPLC. The method of analysis was also validated concerning its linearity, instrumental and method precision (for repeatability, immediate precision and intermediate precision) and accuracy (absolute recovery study). The technique was combined with electrospray mass spectrometry in order to identify the phenolic compounds and the structure of the main phenolics was also confirmed by NMR analysis. The in-house validated HPLC-DAD method showed good linearity for the tested reference compounds as well as satisfactory repeatability and immediate precision values, for both instrument and method. Furthermore, the satisfactory results of intermediate precision analysis and recovery assays indicated that the chromatographic method could be used to quantify the main phenolic compounds of T. x citriodorus with adequate precision and accuracy. The extract was rich in rosmarinic acid (10.4±0.6 mg/g extract) that is a widespread phenolic acid in Thymus plants, but also in luteolin-7-O--glucuronide (12±2 mg/g extract), that was herein reported in Thymus for the first time. Other novel compounds comprised one eriodictyol dihexoside with O-glycosidic linkages, two eriodictyol-O-monohexosides, one quercetagetin dimethyl ether-O-hexoside, one naringenin-O-hexoside and chrysoeriol-7-β-O-glucoside. Having in mind the health-promoting properties reported in literature for some of the main polyphenols found in T. x citriodorus, we suggest that this plant has a high potential for being used as a functional food.por
dc.identifier.citationPereira, Olívia R.; Peres, António M.; Silva, Artur M. S.; Domingues, M. R. M.; Cardoso, Susana M. (2013). Simultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC-UV and ESI- MS combined method. Food Research International . ISSN 0963-9969. 54:2, p. 1773-1780por
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/9497
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Sciencepor
dc.relationSFRH/PROTEC/49600/2009
dc.relationStrategic Project - UI 681 - 2011-2012
dc.subjectThymus x citriodoruspor
dc.subjectPhenolic compoundspor
dc.subjectFlavonoidspor
dc.subjectLuteolin-7-O-a-glucuronidepor
dc.subjectRosmarinic acidpor
dc.subjectMass spectrometrypor
dc.subjectElectrospray ionizationpor
dc.titleSimultaneous characterization and quantification of phenolic compounds in Thymus x citriodorus using a validated HPLC-UV and ESI- MS combined methodpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 681 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FQUI%2FUI0062%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0681%2F2011/PT
oaire.citation.endPage1780por
oaire.citation.startPage1773por
oaire.citation.titleFood Research Internationalpor
oaire.citation.volume54por
oaire.fundingStreamCOMPETE
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira
person.familyNamePeres
person.givenNameOlívia R.
person.givenNameAntónio M.
person.identifier145216
person.identifier107333
person.identifier.ciencia-idB312-AF21-D2FA
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-6275-3134
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridH-1049-2014
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id52264387000
person.identifier.scopus-author-id7102331969
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione8fdab9a-3ad6-47e6-b90c-7a37aabcb453
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isProjectOfPublicationc6cfbfe6-0981-49d7-8a9b-41f9904a0330
relation.isProjectOfPublication762f76ce-d7b9-44dc-870e-3199f6968094
relation.isProjectOfPublication.latestForDiscoveryc6cfbfe6-0981-49d7-8a9b-41f9904a0330

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Simultaneous characterization and quantification of phenolic compounds in T citriodorus.pdf
Size:
388.76 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus