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Prevalence and serotypes of Shiga toxinproducing Escherichia coli (STEC) in dairy cattle from Northern Portugal

2020Journal article Open access
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dc.contributor.authorBallem, Andressa
dc.contributor.authorGonçalves, Soraia
dc.contributor.authorGarcia-Meniño, Isidro
dc.contributor.authorFlament-Simon, Saskia C.
dc.contributor.authorBlanco, Jesús E.
dc.contributor.authorFernandes, Conceição
dc.contributor.authorSaavedra, Maria José
dc.contributor.authorPinto, Carlos
dc.contributor.authorOliveira, Hugo
dc.contributor.authorBlanco, Jorge
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorAlmeida, Carina
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2021-02-27T15:58:37Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2021-02-27T15:58:37Z
dc.date.issued2020
dc.description.abstractThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The prevalence of Shiga toxin (Stx)-producing Escherichia coli (STEC) was determined by evaluating its presence in faecal samples from 155 heifers, and 254 dairy cows in 21 farms at North of Portugal sampled between December 2017 and June 2019. The prevalence of STEC in heifers (45%) was significantly higher than in lactating cows (16%) (p 0.05, Fisher exact test statistic value is 0.00001). A total of 133 STEC were isolated, 24 (13.8%) carried Shiga-toxin 1 (stx1) genes, 69 (39.7%) carried Shiga-toxin 2 (stx2) genes, and 40 (23%) carried both stx1 and stx2. Intimin (eae) virulence gene was detected in 29 (21.8%) of the isolates. STEC isolates belonged to 72 different O:H serotypes, comprising 40 O serogroups and 23 H types. The most frequent serotypes were O29:H12 (15%) and O113:H21 (5.2%), found in a large number of farms. Two isolates belonged to the highly virulent serotypes associated with human disease O157:H7 and O26:H11. Many other bovine STEC serotypes founded in this work belonged to serotypes previously described as pathogenic to humans. Thus, this study highlights the need for control strategies that can reduce STEC prevalence at the farm level and, thus, prevent food and environmental contamination.en_EN
dc.description.sponsorshipThis study was financially supported by: i) project PhageSTEC (POCI-01-0145 -FEDER- 029628) funded by FEDER through COMPETE2020 (Programa Operacional Competitividade e Internacionalização) and by National Funds thought FCT (Fundação para a Ciência e a Tecnologia); ii) strategic project UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by FCT under the scope of the European Regional Development Fund (Norte2020 - Programa Operacional Regional do Norte); iii) project PI16/01477 from Plan Estatal de I+D+I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, Ministerio de Economía y Competitividad (Gobierno de España) and FEDER; and iv) grant ED431C2017/57 from the Consellería de Cultura, Educación e Ordenación Universitaria, (Xunta de Galicia) and FEDER; UIDB/AGR/04033/2020 by National Funds thought FCT. Author IGM acknowledges the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia for the individual grant ED481A-2015/149 and and author SCFS acknowledges the FPU programme for the individual grant FPU15/02644from the Secretaría General de Universidades, Spanish Ministerio de Educación, Cultura y Deporte. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationBallem, Andressa; Gonçalves, Soraia; Garcia-Meniño, Isidro; Flament-Simon, Saskia C.; Blanco, Jesús E.; Fernandes, Conceição; Saavedra, Maria José; Pinto, Carlos; Oliveira, Hugo; Blanco, Jorge; Almeida, Gonçalo; Almeida, Carina (2020). Prevalence and serotypes of Shiga toxinproducing Escherichia coli (STEC) in dairy cattle from Northern Portugal. PLoS ONE. ISSN 1932-6203. 15:12, p. 1-12en_EN
dc.identifier.doi10.1371/journal.pone.0244713en_EN
dc.identifier.urihttp://hdl.handle.net/10198/23351
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.titlePrevalence and serotypes of Shiga toxinproducing Escherichia coli (STEC) in dairy cattle from Northern Portugalen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameFernandes
person.givenNameConceição
person.identifier15483
person.identifier.ciencia-id6514-3D41-4534
person.identifier.orcid0000-0003-2873-501X
person.identifier.scopus-author-id23018336600
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublicationd9c26c54-5e10-4ccb-b7f7-528af9eb9950
relation.isAuthorOfPublication.latestForDiscoveryd9c26c54-5e10-4ccb-b7f7-528af9eb9950

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