Publication
Methyl-end desaturases determine the capability for de novo biosynthesis of polyunsaturated fatty acids in bivalves
| datacite.subject.fos | Ciências Agrárias::Agricultura, Silvicultura e Pescas | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Kabeya, Naoki | |
| dc.contributor.author | Ramos-Llorens, Marc | |
| dc.contributor.author | Nakano, Yo | |
| dc.contributor.author | Gomes-dos-Santos, André | |
| dc.contributor.author | Teixeira, Amilcar | |
| dc.contributor.author | Fujibayashi, Megumu | |
| dc.contributor.author | Haro, Juan G. | |
| dc.contributor.author | Navarro, Juan C. | |
| dc.contributor.author | Castro, L. Filipe C. | |
| dc.contributor.author | Haga, Yutaka | |
| dc.contributor.author | Monroig, Óscar | |
| dc.date.accessioned | 2025-05-29T11:18:53Z | |
| dc.date.available | 2025-05-29T11:18:53Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Recent studies have shown that many invertebrate species possess methyl-end desaturases (herein referred to as ‘ωx’), enabling biosynthesis of polyunsaturated fatty acids (PUFA). However, the phylogenetic distribution of these enzymes across the animal kingdom remains puzzling, possibly due to horizontal gene transfer (HGT) and/ or independent large-scale gene loss in certain invertebrate lineages. In molluscs, ωx genes have been identified in various cephalopods and gastropods but remain barely explored in bivalves. The increasing availability of genomic and transcriptomic resources enables a comprehensive exploration of the ωx gene repertoire in bivalves. To elucidate the distribution of ωx in bivalves, we conducted a broad homology search across existing genome and transcriptome assemblies, followed by functional characterisation of ωx in lineage representative species. Our results revealed no ωx-like sequences in any of the 65 Pteriomorphia species, suggesting gene loss in this clade. However, ωx-like sequences were found in Protobranchia, Palaeoheterodonta and Imparidentia. We analysed ωx from Solemya pusilla (Protobranchia), Lanceolaria oxyrhyncha and Margaritifera margaritifera (Palaeoheterodonta), and Ruditapes philippinarum and Tridacna crocea (Imparidentia). Except for M. margaritifera, which had two ωx genes, each species had a single ωx gene. Functional analysis showed Δ15Δ17Δ19 desaturase activity in the R. philippinarum and T. crocea ωx, while the L. oxyrhyncha ωx exhibited Δ15Δ17 activity but not Δ19. Both ωx from M. margaritifera showed no detectable activity in yeast. Interestingly, the S. pusilla ωx exhibited Δ12 desaturase activity. These findings highlight the diversity of ωx desaturation capabilities in bi- valves, with significant gene loss in Pteriomorphia | eng |
| dc.description.sponsorship | This work was partially supported by JST FOREST Program (Grant Number JPMJFR233H, Japan) and President’s Discretionary Fund: University Reform and Functional Enhancement Project from Tokyo University of Marine Science and Technology. Additionally, this study forms part of the ThinkInAzul programme and was supported by MCIN with funding from the European Union NextGenerationEU (PRTR- C17⋅I1) and by Generalitat Valenciana (THINKINAZUL/2021/26). The Portuguese Foundation for Science and Technology (FCT) funded A.G.S. (2023.07625.CEECIND) and L.F.C.C. (10.54499/CEECINST/00133/201 8/CP1510/CT0004). | |
| dc.identifier.citation | Kabeya, Naoki; Ramos-Llorens, Marc; Nakano, Yo; Gomes-dos-Santos, André; Teixeira, Amilcar; Fujibayashi, Megumu; Haro, Juan G.; Navarro, Juan C.; Castro, L. Filipe C.; Haga, Yutaka; Monroig, Óscar (2025). Methyl-end desaturases determine the capability for de novo biosynthesis of polyunsaturated fatty acids in bivalves. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. ISSN 1388-1981. 1870:5, p. 1-9 | |
| dc.identifier.doi | 10.1016/j.bbalip.2025.159617 | |
| dc.identifier.issn | 1388-1981 | |
| dc.identifier.uri | http://hdl.handle.net/10198/34499 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.ispartof | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Bivalves | |
| dc.subject | Methyl-end desaturase | |
| dc.subject | Biosynthesis | |
| dc.subject | Polyunsaturated fatty acids | |
| dc.title | Methyl-end desaturases determine the capability for de novo biosynthesis of polyunsaturated fatty acids in bivalves | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 9 | |
| oaire.citation.issue | 5 | |
| oaire.citation.startPage | 1 | |
| oaire.citation.title | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | |
| oaire.citation.volume | 1870 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Teixeira | |
| person.givenName | Amilcar | |
| person.identifier | 2153193 | |
| person.identifier.ciencia-id | 9510-3CF3-0393 | |
| person.identifier.orcid | 0000-0001-5336-1174 | |
| person.identifier.scopus-author-id | 7202385393 | |
| relation.isAuthorOfPublication | 4f0753fd-4b4f-46f6-8e2f-20c7f835a32a | |
| relation.isAuthorOfPublication.latestForDiscovery | 4f0753fd-4b4f-46f6-8e2f-20c7f835a32a |