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A prospective study on bioactive properties of wild mushrooms mycelium grown in vitro under different conditions

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dc.contributor.authorSouilem, Fedia
dc.contributor.authorBarros, Lillian
dc.contributor.authorSkhiri, Fethia
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorMartins, Anabela
dc.date.accessioned2016-09-16T15:33:12Z
dc.date.available2016-09-16T15:33:12Z
dc.date.issued2016
dc.description.abstractWild mushrooms have been extensively studied for their value as sources of high quality nutrients and of powerful physiologically bioactive compounds [1,2]. The present study was designed to evaluate the in vitro development of two wild edible mushroom species: Pleurotus eryngii (DC.) Quél. and Suillus belinii (Inzenga) Watling, by testing different solid (Potato Dextrose Agar medium –PDA and Melin-Norkans medium- MMN) and liquid culture media (Potato dextrose broth- PDB and Melin-Norkans medium- MMN). Each strain of mushroom produces a special type of mycelium and this range of characteristics varies in form, color and growth rate. S. bellinii presents a pigmented and rhizomorphic mycelia, whereas, P. eryngii has depigmented and cottony mycelia. The mycelium isolated and grown in PDA showed a faster radial growth compared to the mycelium isolated and grown in both solid and liquid incomplete MMN medium. P. eryngii exhibited a rapid growth and a higher mycelia biomass in both medium compared to S. belinii. Moreover, the obtained mycelia will be characterized in terms of well-recognized bioactive compounds namely, phenolic acids and mycosterols (mainly ergosterol), by using high performance liquid chromatography coupled to diode array and ultraviolet detectors, respectively. These compounds will be correlated to mycelia bioactivity: i) antioxidant activity, evaluated through free radicals scavenging activity, reducing power and lipid peroxidation inhibition in vitro assays; ii) anti-inflammatory activity, assessed through nitric oxide production inhibition in murine macrophages (RAW 264.7 cell line); iii) cytotoxic activity, evaluated either in human tumor cell lines (MCF-7- breast adenocarcinoma, NCIH460- non-small cell lung cancer, HeLa- cervical carcinoma and HepG2- hepatocellular carcinoma) as also in a non-tumor porcine primary liver cells culture established in-house (PLP2). Overall, our expectation is that the bioactive formulations obtained by in vitro culture can be applied as nutraceuticals or incorporated in functional foods.pt_PT
dc.identifier.citationSouilem, Fedia; Barros, Lillian; Skhiri, Fethia; Ferreira, Isabel C.F.R.; Martins, Anabela (2016). A prospective study on bioactive properties of wild mushrooms mycelium grown in vitro under different conditions. In Les Septièmes Journées Scientifiques Internationales sur la Valorisation des Bioressources. Monastir, Tunisiept_PT
dc.identifier.urihttp://hdl.handle.net/10198/13248
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationProviding industry and consumers with new alternative sources of natural additives from plant origin in the classes of preservatives and colouring agents: extraction, purification, stabilization and product development
dc.relationStrategic Project - UI 690 - 2014
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWild mushroomspt_PT
dc.subjectIn vitro culturept_PT
dc.subjectPhenolic acidspt_PT
dc.subjectErgosterolpt_PT
dc.subjectBioactive propertiespt_PT
dc.titleA prospective study on bioactive properties of wild mushrooms mycelium grown in vitro under different conditionspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/107855/2015
oaire.awardNumberPEst-OE/AGR/UI0690/2014
oaire.awardTitleProviding industry and consumers with new alternative sources of natural additives from plant origin in the classes of preservatives and colouring agents: extraction, purification, stabilization and product development
oaire.awardTitleStrategic Project - UI 690 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F107855%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.citation.conferencePlaceTunisiapt_PT
oaire.citation.titleLes Septièmes Journées Scientifiques Internationales sur la Valorisation des Bioressourcespt_PT
oaire.fundingStream5876
person.familyNameBarros
person.familyNameFerreira
person.familyNameMartins
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifier469085
person.identifier144781
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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