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Novel Strategies for Genetically Modified Organism Detection

Publication . Plácido, Alexandra; Amaral, Joana S.; Costa, Joana; Fernandes, Telmo J.R.; Oliveira, Beatriz; Delerue-Matos, Cristina; Mafra, Isabel

All rights reserved. Interest in DNA biosensors for genetically modified organism (GMO) testing is been growing due to their possibility for automation and microfabrication based on simple and portable detection systems. Biosensors can provide rapid, low-cost, sensitive, real-time, and high-throughput measurements suitable for in-field analysis. The efficiency of GMO diagnostics could be improved by simultaneously analyzing several targets, which is presently being exploited using the microarray platforms. As promising alternatives to conventional polymerase chain reaction, isothermal amplification strategies, such as loop-mediated isothermal amplification (LAMP), are low-cost and suitable in-field, enabling visual and electrochemical detection. This chapter intends to provide an overview on the advances in novel biosensing technologies, namely optical, piezoelectric, and electrochemical biosensors, as well as microarrays and LAMP applied to GMO testing.

2015Book part

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