Phytochemical and antioxidant characterization of Brassica oleracea var. costata (tronchuda cabbage) in in vivo and in vitro cultures

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Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential

Publication . Ferreres, Frederico; Sousa, Carla; Valentão, Patrícia; Seabra, Rosa M.; Pereira, J.A.; Andrade, Paula B.

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH- radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.

2007Journal article

Open access

Rumex induratus leaves: phytochemical profiling and antioxidant activity

Publication . Guerra, Luís; Pereira, Carla; Rodrigues, M.A.; Andrade, Paula B.; Gonçalves, Rui; Seabra, Rosa M.; Valentão, Patrícia

Several species of the Rumex (Polygonaceae) genus have been used in traditional medicine, although their high oxalic acid content has been implicated in oxalic intoxication, mainly la children. Rumex induratus Boiss & Reuter is an endemic Liberian herb that prefers rocky habitats of the thermo Mediterranean region.

2008Conference object

Open access

Composição química e actividade antioxidante das folhas internas da couve tronchuda

Publication . Pereira, J.A.; Sousa, Carla; Ferreres, Frederico; Valentão, Patrícia; Seabra, Rosa M.; Andrade, Paula B.

A couve tronchuda (Brassica oleracea L. var. costata DC) é uma das couves mais consumidas pelos portugueses, especialmente durante o Inverno, apresentando as folhas (internas e externas) diferenças organolépticas marcadas. No presente trabalho procedeu-se à caracterização química das folhas internas de couve tronchuda, através da identificação e quantificação dos compostos fenólicos e dos ácidos orgânicos que as compõem e avaliou-se o seu potencial antioxidante. Foram identificados e quantificados, por HPLC-DAD-ESI-MS e HPLC/DAD, respectivamente, 17 compostos fenólicos: quercetina 3-O-soforósido-7-O-glucósido, ácido 3-p-cumaroilquínico, campferol 3-O-soforósido-7-O-glucósido, campferol 3-O-(cafeoil)-soforósido-7-O-glucósido, ácido sinapoilglucósido, campferol 3-O-(sinapoil)-soforósido-7-O-glucósido, campferol 3-O-(feruloil)-soforósido-7-O-glucósido, campferol 3-O-(p-cumaroil)-soforósido-7-O-glucósido, ácido 4-p-cumaroilquínico, ácido sinápico, campferol 3-O-soforósido, 3 isómeros de 1,2-di-sinapoilgentiobiose, 1-sinapoil-2-feruloilgentiobiose, 1,2,2-tri-sinapoilgentiobiose e 1,2’-di-sinapoil-2-feruloilgentiobiose. Foram também identificados e quantificados, por HPLC/UV, sete ácidos orgânicos (ácidos aconítico, cítrico, ascórbico, málico, quínico, chiquímico e fumárico). A actividade antioxidante do extracto aquoso foi aferida pela capacidade para interceptar o radical DPPH e espécies reactivas de oxigénio (radicais superóxido e hidroxilo e ácido hipocloroso), tendo revelado grande efeito protector.

2007Conference object

Open access

Screening of antioxidant compounds during sprouting of Brassica oleracea L. var. costata DC

Publication . Sousa, Carla; Lopes, Graciliana; Pereira, David M.; Taveira, Marcos; Valentão, Patrícia; Seabra, Rosa M.; Pereira, J.A.; Baptista, Paula; Ferreres, Frederico; Andrade, Paula B.

The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.

2007Journal article

Open access

Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC.

Publication . Ferreres, Frederico; Sousa, Carla; Pereira, David M.; Valentão, Patrícia; Taveira, Marcos; Martins, Anabela; Pereira, J.A.; Seabra, Rosa M.; Andrade, Paula B.

The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentrationdependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.

2009Journal article

Restricted access