INOCULAÇÃO DE PLANTAS DE ARROZ COM CIANOBACTÉRIAS FIXADORAS DE AZOTO COM DIFERENTES GRAUS DE TOLERÂNCIA A XENOBIÓTICOS. AVALIAÇÃO DOS MECANISMOS DE TOXICIDADE.

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Fatty acid characterization of cyanobacterial strains isolated from vela lake and mondego river rice fields (central-western, Portugal)

Publication . Galhano, Victor; Santos, Hugo; Geraldes, Ana Maria; Figueiredo, Daniela R.; Crespí, António; Gomes-Laranjo, José; Peixoto, Francisco P.

It is know that cyanobacterial taxonomic groups are characterized by particular lipid patterns that can be used as their biological markers. The present study examined the fatty acid composition of nostocacean heterocystous cyanobacterial strains isolated from Central-western Portuguese freshwater shallow water bodies, namely Vela Lake and rice fields from Mondego River Basin. Morphological characterization showed that strains from Vela Lake belonged to Aphanizomenon gracile (strains UADFA16 and UADFA18), Aphanizomenon flos-aquae (strain UADFA15) and Anabaena cf. solitaria (strain UADFA14) species, whereas rice field strains belonged to Anabaena cylindrica (strain UTAD_A212) and Nostoc muscorum (strain UTAD_N213). Biochemical characterization inferred from lipid analysis showed that predominant fatty acid methyl esters (FAMEs) in the lipids of the strains were palmitic, oleic and α-linolenic, with trace amounts of myristic and C20 polyunsaturated FAMEs. To our knowledge, there is almost no information about lipid composition in freshwater cyanobacterial species living in different habitats in Portugal. Therefore, this limnological study is a contribution to our investigation on freshwater diazotrophic cyanobacteria.

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