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Research Project
alterado para: “Contribuição para o estudo do cancro da próstata: influência do estilo de vida num modelo animal Contribution to the study of prostate cancer: lifestyle influence in an animal model”. Estudo do impacto da atividade física no microambiente do cancro da próstata num modelo animal
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Histological lesions in hpv16-transgenic model: the effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav.
Publication . Ferreira, Tiago; Nascimento-Gonçalves, Elisabete; Moutinho, Magda S.S.; Pires, Maria João; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Costa, Rui Miguel Gil; Rosa, Eduardo; Oliveira, Paula A.
The K14HPV16 mice is a skin squamous carcinoma model that can be
used to test antitumoral properties of several chemical and natural
compounds1. Lavandula penduculata (Mill.) Cav., known as lavender,
belongs to the Laminaceae family and has been used in traditional
medicine as infusions to treat several conditions2. This work aimed to
evaluate the effects of the hydroethanolic French lavender extract (FLE) in
an HPV16-transgenic mice model lesions.
The extract was obtained through a maceration with ethanol/water
(80:20, v/v) and its phenolic composition was determined by HPLC-DADESI/
MS. The FLE was dissolved in drinking water at 6.8 mg/10mL/animal
and the animals were supplemented during 29 consecutive days. Twentyeight
male mice were randomly divided into four groups: (n=7/group):
group I (HPV16- control); II (HPV16- FLE); III (HPV16+ control) and IV
(HPV16+ FLE). After 29 days all animals were sacrificed by xylazineketamine
overdose following cardiac puncture to obtain blood samples.
Skin samples (chest and ear), kidney, liver and spleen were processed for
histological analysis.A total of thirteen compounds were identified in the hydroethanolic
extract, being salvianolic acid B and rosmarinic acid the main molecules
present. Moreover, the compounds revealed to be stable in the drinking
water for 5 days. Histological analyses of skin samples from wild-type
mice exposed (group II) and not exposed (group I) to FLE showed normal
skin histology. Group III showed skin chest epidermal hyperplasia in 100%
of the mice while group IV showed less epidermal hyperplasia frequency
(66.6%) (p>0.05). Concerning to liver, kidney, and spleen lesions there are
no differences between groups (p>0.05). The lavender extract did not
prevent the progression of HPV-16 induced cutaneous lesions in this
model. These data deserve more investigation to clarify the effect of
lavender extract on HPV-16 lesions.
Effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav. On morphometric parameters in HPV-16 trangenic mice
Publication . Nascimento-Gonçalves, Elisabete; Ferreira, Teresa; Moutinho, Magda S.S.; Pires, Maria João; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Costa, Rui Miguel Gil; Oliveira, Paula A.
Lavandula penduculata (Mill.) Cav., common English name French lavender, belongs to the Lamiaceae family and has been used as a medicinal plant in infusions for respiratory and digestive systems and as a therapeutic agent with antiseptic action for cleaning wounds [1,2]. The K14HPV16 mice is a skin squamous carcinoma model that can be used to test the antitumoral properties of several chemical and natural products [3]. The aim of this work was to evaluate the effect of the hydroethanolic French lavender extract (FLE) on body weight, relative organs weights, food and water consumption in an HPV-16-transgenic mice model. The extract was obtained from a maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Twenty-eight male mice were randomly divided into four groups (n=7/group) according to their genotype: group I (HPV16- control); II (HPV16- FLE); III (HPV16+ control) and IV (HPV16+ FLE). The FLE was administrated orally in drinking water at 6.8 mg/10mL/animal to animals from groups II and IV and changed every 4 days. The animals were kept under controlled conditions such as temperature, light and humidity
Effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav. On morphometric parameters in HPV-16 trangenic mice
Publication . Nascimento-Gonçalves, Elisabete; Ferreira, Teresa; Moutinho, Magda S.S.; Pires, Maria João; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Costa, Rui Miguel Gil; Oliveira, Paula A.
Lavandula penduculata (Mill.) Cav., common English name French lavender, belongs to the Lamiaceae family and has been used as a medicinal plant in infusions for respiratory and digestive systems and as a therapeutic agent with antiseptic action for cleaning wounds [1,2]. The K14HPV16 mice is a skin squamous carcinoma model that can be used to test the antitumoral properties of several chemical and natural products [3]. The aim of this work was to evaluate the effect of the hydroethanolic French lavender extract (FLE) on body weight, relative organs weights, food and water consumption in an HPV-16-transgenic mice model. The extract was obtained from a maceration with ethanol/water (80:20, v/v), and the phenolic composition was determined through HPLC-DAD-ESI/MS. Twenty-eight male mice were randomly divided into four groups (n=7/group) according to their genotype: group I (HPV16- control); II (HPV16- FLE); III (HPV16+ control) and IV (HPV16+ FLE). The FLE was administrated orally in drinking water at 6.8 mg/10mL/animal to animals from groups II and IV and changed every 4 days. The animals were kept under controlled conditions such as temperature, light and humidity
Toxicological and anti-tumor effects of a linden extract (Tilia platyphyllos Scop.) in a HPV16-transgenic mouse model
Publication . Patwal, Tiago; Nascimento-Gonçalves, Elisabete; Macedo, Sara; Borges, Inês; Gama, Adelina; Costa, Rui Miguel Gil; Neuparth, Maria João; Lanzarin, Germano; Venâncio, Carlos; Félix, Luís; Gaivão, Isabel; Alvarado, Antonieta; Pires, Maria João; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Rosa, Eduardo; Oliveira, Paula A.
Tilia platyphyllos Scop. is a popular broad-leaved tree, native to Central and Southern Europe. Hydroethanolic extracts rich in phenolic compounds obtained from T. platyphyllos Scop. have shown in vitro antioxidant, anti-inflammatory and antitumor properties. The aim of this work was to evaluate the therapeutic properties of a hydroethanolic extract obtained from T. platyphyllos in HPV16-transgenic mice. The animals were divided into eight groups according to their sex and phenotype. Four groups of female: HPV+ exposed to linden (HPV linden; n = 6), HPV+ (HPV water; n = 4), HPV- exposed to linden (WT linden; n = 5) and HPV- (WT water; n = 4) and four groups of male: HPV+ exposed to linden (HPV linden; n = 5), HPV+ (HPV water; n = 5), HPV- exposed to linden (WT linden; n = 5) and HPV- (WT water; n = 7). The linden (Tilia platyphyllos Scop.) extract was orally administered at a dose of 4.5 mg/10 mL per animal (dissolved in water) and changed daily for 33 days. The hydroethanolic extract of T. platyphyllos consisted of protocatechuic acid and (-)-epicatechin as the most abundant phenolic acid and flavonoid, respectively, and was found to be stable during the studied period. In two male groups a significant positive weight gain was observed but without association with the linden extract. Histological, biochemical, and oxidative stress analyses for the evaluation of kidney and liver damage support the hypothesis that the linden extract is safe and well-tolerated under the present experimental conditions. Skin histopathology does not demonstrate the chemopreventive effect of the linden extract against HPV16-induced lesions. The linden extract has revealed a favourable toxicological profile; however, additional studies are required to determine the chemopreventive potential of the linden extract. This journal is
Therapeutic properties of Tilia platyphyllos Scop. in K14HPV16 mice
Publication . Ferreira, Tiago; Nascimento-Gonçalves, Elisabete; Gama, Adelina; Costa, Rui Miguel Gil; Neuparth, Maria João; Lanzarin, Germano; Venâncio, Carlos; Félix, Luís; Pires, Maria João; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Rosa, Eduardo; Oliveira, Paula A.
Tila platyphyllos Scop. is a popular broad-leave tree, native to central and southern Europe
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
FARH
Funding Award Number
SFRH/BD/136747/2018