AUTENTICAÇÃO GEOGRÁFICA DO MEL E PROPOLIS PORTUGUÊS PELA CARACTERIZAÇÃO QUÍMICA DO PERFIL FENÓLICO. POTENCIALIDADES ANTIOXIDANTES

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Propolis phenolic profile: a study in the atlantic islands of Azores

Publication . Falcão, Soraia; Cardoso, Susana M.; Domingues, Maria R.M.; Freire, Cristina; Vilas-Boas, Miguel

This work outlines a characterization of the phenolic composition of the Azorean islands propolis. For that, eleven samples from S. Miguel and Terceira islands, were extracted and characterized. According to the I-IPLC analysis, an samples showed a similar phenolic profile, with 37 compounds identified by ESI-MS analysis and UV spectra. Although the samples revealed a close phenolic composition, significant differences were found in their concentrations, probably due to variations in flora distribution around the beehive, and therefore, in resin availability for bees.

2010Conference paper

Open access

Phenolic quantification and botanical origin of Portuguese propolis

Publication . Falcão, Soraia; Tomás, Andreia; Vale, Nuno; Gomes, Paula; Freire, Cristina; Vilas-Boas, Miguel

The production of propolis by honeybees results from a selective collection of exudates from leaf buds and plants present in the hive neighborhood leading to a resin with many potentialities in the pharmaceutical industry. This study aims to quantify the phenolic content in propolis from different Portuguese regions and in the potential floral sources, Populus x Canadensis Moench buds and Cistus ladanifer L., in order to establish links with geographical and botanical origin. The Portuguese propolis revealed a phenolic profile with marked differences in concentrations: the richness in flavonoids is common in all regions, but more evident in propolis from central interior, south and Madeira. The composition of poplar type propolis common in temperate zones was observed in the north, central coast and Azores, while the central interior and south samples, with a composition rich in kaempferol derivatives, resemble the C. ladanifer exudates, a spontaneous bush widespread in the Mediterranean. The compound kaempferol-3,7-dimethyl-ether, absent in the poplar type propolis, can be regard as a possible marker for the discrimination of these two types of propolis. (C) 2013 Elsevier B.V. All rights reserved.

2013Journal article

Open access

Propolis phenolic profile: a study in the Atlantic islands of Azores

Publication . Falcão, Soraia; Cardoso, Susana M.; Domingues, Maria R.M.; Freire, Cristina; Vilas-Boas, Miguel

Propolis it is a natural resinous substance that honeybees (Apis melifera, L.) collect from buds and exudates of plants and transformed in the presence of bee enzymes. This substance plays an important role in the hive as a construction and defence material due to its chemical and biological properties.[1] Propolis composition is extremely complex and dependent on its vegetal source, geographical location and climatic conditions. Typically this natural product includes resin (50%), beeswax (30%), essential oils (10%), pollen (5%) and other organic compounds (5%).[2] Due to the abundance in phenolic compounds (flavonoids, phenolic acids and their esters) and to its biological and pharmacological properties this group of substances became the target of many research studies in propolis. This work outlines an extensive qualitative and quantitative characterization of the phenolic composition of Azorean islands propolis. For that, eleven samples of propolis, from S. Miguel and Terceira island, were extracted and characterized according to the previous work.[3] The HPLC results show a similar chromatographic profile for all samples, despite their origin, with 37 compounds identified by ESI-MS/MSn analysis and confirmed by HPLC-DAD. Although the samples revealed a similar phenolic composition, significant differences in the concentrations of the different compounds were found, probably due to the differences in flora distribution around the beehive, and therefore resin availability for bees.

2010Conference object

Open access

Characterization of new phenolic derivatives in Portuguese propolis by electrospray mass spectroscopy

Publication . Falcão, Soraia; Vilas-Boas, Miguel; Freire, Cristina; Domingues, Maria R.M.; Cardoso, Susana M.

This research outlines an extensive characterization of the phenolic composition of a propolis sample from the northeast of Porto gal. For that, ao ethanolic extract was prepared, fractionated by HPLC and the identification of the phenolic compounds was done by electro spray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds in the Portoguese propolis sample, including seven that were described for the first time. Two of these new compounds had [M-Hr ions at mlz 403, aod the others had [M-Hr ions at mlz 433, mlz 461, mlz 417, mlz 475, and mlz 565. In general, the molecular weight of these compounds was higher thao the common phenolic compounds of propolis aod their fragmentation pattern suggested that they belong to the flavonoid family probably lioked with a phenylpropanoic acid moiety in the position C5 (mlz 403, 433, 461, 475) and C3 (mlz 403, 417), corresponding to pinocembrio and pinobaoksin derivatives. The ion at mlz 565 seems to be a p-counlaric ester derivative dimer.

2009Conference paper

Open access

The volatile composition of Portuguese propolis towards its origin discrimination

Publication . Falcão, Soraia; Freire, Cristina; Figueiredo, Ana Cristina; Vilas-Boas, Miguel

The volatiles from thirty six propolis samples collected from six different geographical locations in Portugal (mainland, Azores archipelago and Madeira Island) were evaluated. Populus x canadensis Moenchen leaf-buds and Cistus ladanifer L. branches essential oils were comparatively analysed. The essential oils were isolated by hydrodistillation and analysed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS). Cluster analysis based on propolis samples volatiles chemical composition defined three main clusters, not related to sample site collection. Cluster I grouped 28 samples with high relative amounts of oxygen-containing sesquiterpenes (20-77%), while cluster II grouped 7 samples rich in oxygen-containing monoterpenes (9-65%) and the only sample from cluster III was monoterpene hydrocarbons rich (26%). Although Populus x canadensis and Cistus ladanifer were associated as resin sources of Portuguese propolis, other Populus species as well as plants like Juniperus genus may contribute to the resin in specific geographical locations.

2015Journal article

Open access