Percorrer por autor "Souza, Gustavo Henrique"
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- Inhibitory Effects of Aqueous and Hydroalcoholic Extracts from Jatobá Coat (Hymenaea courbaril L.) on Pancreatic Amylase and Starch AbsorptionPublication . Polo, Ana Caroline; Uber, Thaís Marques; Souza, Gustavo Henrique; Corrêa, Rúbia C.G.; Santos Filho, José Rivaldo dos; Sá-Nakanishi, Anacharis Babeto de; Seixas, Flávio Augusto Vicente; Bracht, Adelar; Peralta, Rosane MarinaJatobá (Hymenaea courbaril) is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá. Within this context, the present work attempts to find possible applications for the jatobá coat in glycemic control through inhibition of α-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an α-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited α-amylase. The IC50 values for the aqueous and hydroalcoholic extracts were 81.98 ± 3.53 µg/mL and 51.06 ± 0.42 µg/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the α-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá coat warrant further investigations as potential modulators of glycemia following starch ingestion.
- Tamarindus indica sub-products as potential tools for simultaneous management of diabetes and obesityPublication . Souza, Gustavo Henrique; Silva, Beatriz Paes; Viana Neto, Gabriel Arcanjo; Finimundy, Tiane C.; Santos, Thalita Faleiro Demito; Bueno, Paulo Sergio Alves; Souza, Felipe de Oliveira; Pilau, Eduardo J.; Barros, Lillian; Comar, Jurandir F.; Bracht, Livia; Peralta, Rosane M.; Bracht, Adelar; Sá-Nakanishi, Anacharis B.Aiming at valorizing industrial wastes of tamarind, the present work evaluated the actions of seed, leaf and peel extracts on starch and fat absorption through starch and triglyceride tolerance tests in mice. The actions of all extracts on the α-amylase and lipase were also characterized using classical kinetic assays. All extracts inhibited starch digestion in vivo, but the seed extract was the most effective one with an ID50 of 151.4 mg kg−1. The mechanism behind this inhibition probably involves the pancreatic α-amylase, which was strongly inhibited by the seed extract under in vitro conditions (IC50 = 13.3 μg mL−1) and much less strongly by the leaf and peel extracts (IC50 values in the vicinity of 400 μg mL−1). The pancreatic lipase, conversely, was inhibited solely by the seed extract, with an IC50 of 31.5 μg mL−1. As a consequence of this property, the seed extract also inhibited triglyceride absorption, as indicated by olive oil tolerance tests, which revealed an ID50 of 214.9 mg kg−1. The seed extract also possessed the strongest antioxidant capacity and the highest content in phenolic groups. Chemical analyses revealed that all extracts present a great variety of phenolic compounds and that the seed extract possesses at least 5 unique compounds (ursodiol, masoprocol, lenticin, chenodeoxycolic acid and 13-keto-9Z,11E-octadecadienoic acid), which, according to docking studies, could be involved in the inhibition of both α-amylase and lipase. The overall conclusion is that the tamarind seed extract displays great potential for being used in the management of obesity and diabetes.