Percorrer por autor "Silva, Gabriela Catuzo Canonico"
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- Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harmsPublication . Silva, Gabriela Catuzo Canonico; Machado, Mariane de Almeida; Sakumoto, Karina; Inumaro, Rodrigo Sadao; Gonçalves, José Eduardo; Mandim, Filipa; Vaz, Josiana A.; Valle, Juliana Silveira do; Faria, Maria Graciela Iecher; Ruiz, Suelen Pereira; Piau Junior, Ranulfo; Gonçalves, Daniela Dib; Gazim, Zilda CristianiGallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.
- Exploring the Potential of Hop (Humulus lupulus) Cone Residue: Chemical Characterization and Evaluation of BioactivitiesPublication . Reyes, Giulia Boito; Santos, Emylaine Pereira dos; Santos, Everton da Silva; Gonçalves, Laura Correia; Silva, Gabriela Catuzo Canonico; Gazim, Zilda Cristiani; Gonçalves, Regina Aparecida Correia; Oliveira, Arildo José Braz de; Pinela, José; Mandim, Filipa; Pires, Tânia C.S.P.; Cardozo-Filho, Lucio; Corrêa, Rúbia C.G.; Gonçalves, José EduardoHumulus lupulus L. (hops), belonging to the Cannabaceae family, is grown mainly for brewing, with 98% of global production directed to this sector. Moreover, large volumes of female cone residues are generated as by-products, representing a valuable source of bioactive compounds that can be valorized under green chemistry principles. This study aimed to extract bioactive compounds from hop cone residues sourced from craft breweries using ultrasound-assisted (EH-UA) and microwave-assisted (EH-MA) extraction methods. Hydroalcoholic extracts (70%) were analyzed for chemical composition, antioxidant, antimicrobial, antiproliferative, nitric oxide (NO)-production inhibition, and photoprotective activities. GC-MS identified 32 compounds in EH-MA and 30 in EH-UA, including terpenes, sesquiterpenes, oxygenated sesquiterpenes, and fatty acids. Both extracts demonstrated strong antioxidant activity in cell-based (TBARS, OxHLIA) and chemical (DPPH, ABTS, FRAP) assays, particularly EH-MA. Significant antibacterial activity was observed, especially against Enterobacter cloacae, Pseudomonas aeruginosa, and Staphylococcus aureus (MIC 1–10 mg/mL), as well as antifungal activity against Aspergillus brasiliensis (MIC 2–2.5 mg/mL). Selective antiproliferative activity was observed against tumor cell lines Caco-2 and MCF-7 (GI50 25 μg/mL), without cytotoxicity toward nontumor cell lines Vero and PLP2 (GI50 > 400 μg/mL). All extracts inhibited the production of the inflammation mediator NO, with EH-MA showing the most potent effect (IC50 of 35 μg/mL), followed by EH-UA (IC50 of 55 μg/mL). Photoprotective potential was also demonstrated, with SPF values of 19 (EH-MA) and 18 (EH-UA). In conclusion, hop cone residues can yield multifunctional extracts with antioxidant, antimicrobial, antiproliferative, anti-inflammatory, and photoprotective activities, which support their sustainable upcycling for pharmacological, nutraceutical, and cosmetic applications.