Logo do repositório

Percorrer por autor "Santos, C.R."

A mostrar 1 - 2 de 2
  • Antibacterial activity of Euterpe oieracea Mart. seeds against clinical isolates
    Publication . Alves, Maria José; Pimentel, Maria Helena; Baía, Lilia; Soares, R.P.; Santos, C.R.; Regis, Wiliam C.B.; Ferreira, Isabel C.F.R.
    Introduction: The use of antibiotics – including the over- and misuse – in human and veterinary practices selected for resistant pathogens led to their emergence and dissemination along with the transmission of resistant bacteria [1]. Due to this problem, there is a need to investigate new strategies to reverse this tendency in order to achieve the appropriate and effective treatment against infections. Aims: The present work aimed to evaluate the antibacterial activity of an aqueous extract prepared from Euterpe Oleracea Mart. (açai) seeds (originated from Brazil [2]), against clinical isolates from “Centro Hospitalar de Trás-os-Montes e Alto Douro- Unidade de Vila Real”, with high resistance profiles to different antibiotics. Material and Methods: The minimal inhibitory concentrations (MIC) of the growth of Gram-positive and Gram-negative bacteria were determined by the colorimetric assay based on the use of p-iodonitrotetrazolium chloride (INT) dye, after application of the microdilution method. Results and discussion: The açaí aqueous extract showed antimicrobial activity against all the tested Gram-positive bacteria. The lowest MICs (0.25 mg/ml) were obtained against Methicillin Susceptible Staphylococcus aureus (MSSA) and Methicillin Resistant S. aureus (MRSA), followed by the MIC 0.5 mg/ml correspondent to Streptococcus agalactiae and Enterococcus faecalis. Regarding the inhibition of the growth of Gram-negative bacteria, the extract was active against Morganella morganii, Pseudomonas aeruginosa and Acinetobacter baumannii with MICs 1 mg/ml e 2 mg/ml, respectively. The results were better for Gram-positive bacteria in comparison with Gram-negative bacteria. Conclusion: However, even at low concentration (2 mg/ml) the results are very promising for Gram-negative bacteria with high resistance profiles and commonly related with health care-associated infections, namely Pseudomonas aeruginosa and Acinetobacter baumannii.
    2015Documento de conferência Acesso aberto Ver mais
  • Mycotoxigenic potential of fungi isolated from highly cured Portuguese cheese
    Publication . Dias, Teresa; Santos, V.A.; Santos, C.R.; Rodrigues, Pedro João; Venâncio, Armando
    The cheese industry in Portugal offers a plethora of high-quality products. The São Jorge cheese is an example and has obtained the Protected Designation of Origin (PDO) certification in 1986. This cheese has long ripening periods of up to 36 months, which raises concerns regarding food losses and health risks due to fungal proliferation. In this study, the mycobiota of three São Jorge cheese samples with different ripening periods (five, nine and thirty months) was studied to predict the associated mycotoxigenic risk. From the three cheese samples, 76 fungal isolates were identified through molecular methods (analysis of ITS and/or partial benA). Penicillium spp. ser. Camembertiorum, mainly P. solitum and P. echinulatum, were present in all the analyzed cheeses. Scopulariopsis spp. and some yeasts (predominantly Saccharomyces cerevisiae) were also part of the mycobiota of the cheeses. Although none of these species is a common producer of mycotoxins, an analysis of the cheese by mass spectrometry will be carried out. Furthermore, the overall mycobiota will be studied through metabarcoding to uncover the presence of potential mycotoxigenic species that have not been isolated through the culturomics approach
    2023Documento de conferência Acesso aberto Ver mais