Percorrer por autor "Pinto, Pedro"
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- 5th Symposium of Applied Science for Young Researchers: Book of AbstractsPublication . Fernandes, Florbela P.; Torres, Helena; Pinto, Pedro; Malta, SilvestreThese are the abstracts of the 5th Symposium of Applied Science for Young Researchers – SASYR. This scientific event welcomed scientific works on the topics covered by the following four research centers: – CeDRI (from IPB, Instituto Politécnico de Bragança) – 2Ai (from IPCA, Instituto Politécnico do Cávado e do Ave) – GECAD (from IPP, Instituto Politécnico do Porto) – ADiT-lab (from IPVC, Instituto Politécnico de Viana do Castelo) The primary objective of SASYR 2025 is to create a welcoming and relaxed environment for young researchers to present their work, discuss recent findings, and explore new ideas. In this way, this event offers an opportunity for the CeDRI, 2Ai, GECAD, and ADiT-lab research communities to leverage synergies and promote collaborations, thereby enhancing the quality of their research. The SASYR 2025 took place at Instituto Politécnico de Viana do Castelo, Viana do Castelo, Portugal, on 2 July 2025.
- 5th Symposium of Applied Science for Young Researchers: ProceedingsPublication . Fernandes, Florbela P.; Torres, Helena; Pinto, Pedro; Malta, SilvestreThis document presents the proceedings of the 5th Symposium of Applied Science for Young Researchers – SASYR 2025. This scientific event welcomed scientific works on the topics covered by the following four research centers: – CeDRI (from IPB, Instituto Politécnico de Bragança) – 2Ai (from IPCA, Instituto Politécnico do Cávado e do Ave) – GECAD (from IPP, Instituto Politécnico do Porto) – ADiT-lab (from IPVC, Instituto Politécnico de Viana do Castelo) The primary objective of SASYR 2025 is to create a welcoming and relaxed environment for young researchers to present their work, discuss recent findings, and explore new ideas. In this way, this event offers an opportunity for the CeDRI, 2Ai, GECAD, and ADiT-lab research communities to leverage synergies and promote collaborations, thereby enhancing the quality of their research. The SASYR 2025 took place at Instituto Politécnico de Viana do Castelo, Viana do Castelo, Portugal, on 2 July 2025.
- Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia coli Linked to Sternal Bursitis in Chickens: A One Health PerspectivePublication . Ribeiro, Jessica; Silva, Vanessa; Freitas, Catarina; Pinto, Pedro; Vieira-Pinto, Madalena; Batista, Rita; Nunes, Alexandra; Gomes, João Paulo; Pereira, José Eduardo; Igrejas, Gilberto; Barros, Lillian; Heleno, Sandrina A.; Reis, Filipa S.; Poeta, PatríciaSternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 Escherichia coli isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, including ST155, ST201, and ST58. Resistance to tetracycline and ciprofloxacin was common, and several isolates carried genes encoding beta-lactamases, including blaTEM-1B. Chromosomal mutations associated with quinolone and fosfomycin resistance (e.g., gyrA p.S83L, glpT_E448K) were also identified. WGS revealed a high number of virulence-associated genes per isolate (58-96), notably those linked to adhesion (fim, ecp clusters), secretion systems (T6SS), and iron acquisition (ent, fep, fes), suggesting strong pathogenic potential. Many isolates harbored virulence markers typical of ExPEC/APEC, such as iss, ompT, and traT, even in the absence of multidrug resistance. Our findings suggest that E. coli from sternal bursitis may act as reservoirs of resistance and virulence traits relevant to animal and public health. This highlights the need for including such lesions in genomic surveillance programs and reinforces the importance of integrated One Health approaches.
- Genomic and phenotypic characterization of Enterococcus faecalis from broiler sternal bursitis: antimicrobial resistance and one health risksPublication . Ribeiro, Jessica; Silva, Vanessa; Pinto, Pedro; Vieira-Pinto, Madalena; Batista, Rita; Nunes, Alexandra; Gomes, João Paulo; Igrejas, Gilberto; Barros, Lillian; Heleno, Sandrina A.; Reis, Filipa S.; Poeta, PatríciaEnterococcus spp. are opportunistic bacteria capable of acquiring antimicrobial resistance and virulence traits, facilitating their adaptation to multiple ecological niches. Sternal bursitis, a condition affecting poultry welfare and carcass quality, remains poorly characterized from a microbiological perspective. This study provides the first genomic and phenotypic characterization of Enterococcus isolates from bursitis lesions in broilers, aiming to assess their antimicrobial resistance profiles, virulence determinants, and genetic diversity within a One Health framework. A total of 44 Enterococcus isolates were recovered from 48 sternal bursitis lesions, all identified as E. faecalis. Resistance was common for tetracycline (70.5%) and erythromycin (27.3%), while all isolates remained susceptible to critically important antimicrobials, including vancomycin and linezolid. Whole genome sequencing revealed a genetically diverse population, comprising multiple sequence types, plasmid replicons, and virulence gene profiles, including determinants for adhesion, biofilm formation, capsule synthesis, and extracellular proteases. Ionophore resistance genes (narA, narB) were also detected in several lineages. The coexistence of antimicrobial resistance and virulence determinants, often linked to mobile genetic elements, highlights the potential of lesion-associated with E. faecalis to act as reservoirs of relevant genes with zoonotic implications. Overall, this work highlights the diverse and ecological role of Enterococcus in extraintestinal poultry infections, reinforcing the need for continued genomic surveillance to promote animal health, food safety, and antimicrobial stewardship.
- Verificação da segurança contra o fogo da Escola Superior de Tecnologia e Gestão do Instituto Politécnico de Bragança- medidas de autoproteçãoPublication . Cardoso, Leocádia Helena Patrício; Pinto, Pedro; Ferreira, Débora; Fernandes, SílviaDe acordo com a nova legislação de Segurança contra Incêndio em Edifícios (Decreto – Lei 220/2008 de 12/11 e Portaria 1532/2008 de 29/12) (1), torna-se necessário efetuar um levantamento das soluções e medidas existentes para o edifício da Escola Superior de Tecnologia e Gestão do Instituto Politécnico de Bragança, enquadrá-las e verificar a sua aplicabilidade de acordo com a legislação bem como apresentar propostas para a sua otimização.