Percorrer por autor "Peralta, Rosane Marina"
A mostrar 1 - 3 de 3
Resultados por página
Opções de ordenação
- Encapsulation in Polyvinylpyrrolidone Protects the Ora-Pro-Nobis (Pereskia Aculeata Miller) Extracts against the Deleterious effects of In Vitro Gastrointestinal DigestionPublication . Teixeira, Valéria Maria Costa; Oliveira, Anielle de; Santos Filho, José Rivaldo dos; Carvalho, Amarilis Santos de; Uchoa, Ashley; Peron, Ana Paula; Mandim, Filipa; Pereira, Eliana; Leimann, Fernanda Vitoria; Contato, Alex Graça; Peralta, Rosane MarinaPereskia aculeata Miller, commonly known as ora-pro-nobis, is a South American plant widely found in Brazil and classified as a wild plant. Its leaves are rich in bioactive compounds with reported antioxidant, antimicrobial, and anti-inflammatory properties, supporting growing interest in their use as functional ingredients. This study compared conventional hydroethanolic extraction of antioxidant compounds from P. aculeata leaves with a process combining extraction and simultaneous encapsulation, aiming to evaluate differences in stability and bioaccessibility during simulated gastrointestinal digestion. Free and encapsulated extracts (80:20 ethanol: water, v/v) were characterized by Fourier-transform infrared spectroscopy (FTIR), while encapsulated systems were further analyzed by dynamic light scattering (DLS), atomic force microscopy (AFM), and transmission electron microscopy (TEM). Thermal behavior and antiproliferative activity were also evaluated. Total phenolics, flavonoids and antioxidant activity were determined before and after in vitro digestion. Extraction and encapsulation yields were 17.25% and 92.75%, respectively, with the encapsulated extract corresponding to 62% of the free extract. Simulated digestion reduced phenolic content, flavonoid levels, and antioxidant activity in both systems; however, significantly lower losses were observed for the encapsulated extract, indicating improved stability. The apparent divergence between better radical-scavenging stability (ABTS/DPPH) and lower TPC/TFC bioaccessibility in the encapsulated samples likely reflects release- and assay-specific effects. PVP can establish hydrogen-bond and dipolar interactions with phenolics, which may slow their diffusion or release and reduce their immediate availability to react with the Folin-Ciocalteu and aluminum-chloride reagents, lowering measured TPC/TFC in the digesta fraction. In contrast, encapsulation can limit oxidative degradation during digestion, thereby preserving redox-active constituents and sustaining radical-scavenging capacity in ABTS/DPPH assays.
- Inhibitory Effects of Aqueous and Hydroalcoholic Extracts from Jatobá Coat (Hymenaea courbaril L.) on Pancreatic Amylase and Starch AbsorptionPublication . Polo, Ana Caroline; Uber, Thaís Marques; Souza, Gustavo Henrique; Corrêa, Rúbia C.G.; Santos Filho, José Rivaldo dos; Sá-Nakanishi, Anacharis Babeto de; Seixas, Flávio Augusto Vicente; Bracht, Adelar; Peralta, Rosane MarinaJatobá (Hymenaea courbaril) is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá. Within this context, the present work attempts to find possible applications for the jatobá coat in glycemic control through inhibition of α-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an α-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited α-amylase. The IC50 values for the aqueous and hydroalcoholic extracts were 81.98 ± 3.53 µg/mL and 51.06 ± 0.42 µg/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the α-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá coat warrant further investigations as potential modulators of glycemia following starch ingestion.
- Persistence of antioxidant, antimutagenic, and antigenotoxic properties of Polygonum maritimum L. extract after in vitro digestion supports its high potential as a functional food ingredientPublication . Oliveira, Daniela; Hayrapetyan, Ruzanna; Dias, Maria Inês; Rodrigues, Maria João; Correa, Vanesa Gesser; Carvalho, António Paulo; Corre, Ludovic Le; Séverin, Isabelle; Peralta, Rosane Marina; Santos, Miguel Machado; Custódio, Luísa; Chagnon, Marie-Christine; Oliveira, RuiContinuous exposure to xenobiotic-contaminated food causes adverse effects that may lead to health complications, including cancer. Halophytes are rich in polyphenols that exhibit notable biological properties and may contribute to cancer prevention. However, digestion can modify the chemical structure of polyphenols, potentially reducing their biological properties. This study investigated the protective effects of a polyphenol-rich extract from the halophyte Polygonum maritimum L. (PME) against contaminants-induced toxicity and assessed the persistence of these properties following in vitro digestion. LC-DAD-ESI/MSn analysis revealed that the phenolic composition of PME decreased considerably after digestion. Nevertheless, the antioxidant activity of PME, measured as a decrease in reactive oxygen species levels in H2O2-challenged HepG2 cells, persisted post-digestion. The extract showed enhanced anti-inflammatory activity after digestion, as shown by the ability to reduce (NO)-N-center dot production in lipopolysaccharide-stimulated RAW 264.7 cells. The antigenotoxicity of PME against the contaminant benzo[a]pyrene (BaP) in Caco-2 and HepG2 cells remained significant following upper gastrointestinal tract digestion (DPME), whereas the antigenotoxicity against H2O2 only persisted in Caco-2 cells. After colonic fermentation (FPME), antigenotoxicity was observed against H2O2 in HepG2 cells. PME also displayed antimutagenicity towards BaP in Salmonella typhimurium TA98 and TA100 strains, which remained relevant in DPME, but was drastically reduced in FPME. Furthermore, Danio rerio fed with PME and BaP showed reduced negative effects in terms of size and lipid peroxidation. These results suggest that PME can protect against contaminants-induced toxicity along the gastrointestinal tract, maintaining bioactivity until colonic fermentation. Thus, PME can be a promising functional food ingredient for health improvement.