Percorrer por autor "Peralta, Rosane Marina"
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- Encapsulation in Polyvinylpyrrolidone Protects the Ora-Pro-Nobis (Pereskia Aculeata Miller) Extracts against the Deleterious effects of In Vitro Gastrointestinal DigestionPublication . Teixeira, Valéria Maria Costa; Oliveira, Anielle de; Santos Filho, José Rivaldo dos; Carvalho, Amarilis Santos de; Uchoa, Ashley; Peron, Ana Paula; Mandim, Filipa; Pereira, Eliana; Leimann, Fernanda Vitoria; Contato, Alex Graça; Peralta, Rosane MarinaPereskia aculeata Miller, commonly known as ora-pro-nobis, is a South American plant widely found in Brazil and classified as a wild plant. Its leaves are rich in bioactive compounds with reported antioxidant, antimicrobial, and anti-inflammatory properties, supporting growing interest in their use as functional ingredients. This study compared conventional hydroethanolic extraction of antioxidant compounds from P. aculeata leaves with a process combining extraction and simultaneous encapsulation, aiming to evaluate differences in stability and bioaccessibility during simulated gastrointestinal digestion. Free and encapsulated extracts (80:20 ethanol: water, v/v) were characterized by Fourier-transform infrared spectroscopy (FTIR), while encapsulated systems were further analyzed by dynamic light scattering (DLS), atomic force microscopy (AFM), and transmission electron microscopy (TEM). Thermal behavior and antiproliferative activity were also evaluated. Total phenolics, flavonoids and antioxidant activity were determined before and after in vitro digestion. Extraction and encapsulation yields were 17.25% and 92.75%, respectively, with the encapsulated extract corresponding to 62% of the free extract. Simulated digestion reduced phenolic content, flavonoid levels, and antioxidant activity in both systems; however, significantly lower losses were observed for the encapsulated extract, indicating improved stability. The apparent divergence between better radical-scavenging stability (ABTS/DPPH) and lower TPC/TFC bioaccessibility in the encapsulated samples likely reflects release- and assay-specific effects. PVP can establish hydrogen-bond and dipolar interactions with phenolics, which may slow their diffusion or release and reduce their immediate availability to react with the Folin-Ciocalteu and aluminum-chloride reagents, lowering measured TPC/TFC in the digesta fraction. In contrast, encapsulation can limit oxidative degradation during digestion, thereby preserving redox-active constituents and sustaining radical-scavenging capacity in ABTS/DPPH assays.
- Inhibitory Effects of Aqueous and Hydroalcoholic Extracts from Jatobá Coat (Hymenaea courbaril L.) on Pancreatic Amylase and Starch AbsorptionPublication . Polo, Ana Caroline; Uber, Thaís Marques; Souza, Gustavo Henrique; Corrêa, Rúbia C.G.; Santos Filho, José Rivaldo dos; Sá-Nakanishi, Anacharis Babeto de; Seixas, Flávio Augusto Vicente; Bracht, Adelar; Peralta, Rosane MarinaJatobá (Hymenaea courbaril) is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá. Within this context, the present work attempts to find possible applications for the jatobá coat in glycemic control through inhibition of α-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an α-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited α-amylase. The IC50 values for the aqueous and hydroalcoholic extracts were 81.98 ± 3.53 µg/mL and 51.06 ± 0.42 µg/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the α-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá coat warrant further investigations as potential modulators of glycemia following starch ingestion.