Percorrer por autor "Patto, Maria Carlota Vaz"
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- Differential regulation of antioxidant machinery on grass pea partial resistance against powdery mildew and rust pathogensPublication . Martins, Davide Coelho; Mendes, Francisco A.; Araújo, Susana de Sousa; Rubiales, Diego; Patto, Maria Carlota VazPowdery mildew (Erysiphe pisi, E. trifolii) and rust (Uromyces pisi) are important diseases affecting grass pea (Lathyrus sativus). This study investigates grass pea’s histological, enzymatic, and metabolic responses to these pathogens using accessions with contrasting resistance. Partially resistant (PR) accessions exhibited smaller fungal colonies from 48 h after inoculation (HAI) onwards. Enhanced superoxide dismutase (SOD) activity was observed as early as 12 HAI in PR accessions against both powdery mildews, associated with increased ascorbate peroxidase (APX) and catalase (CAT) activity in E. trifolii and E. pisi infections, respectively. Moreover, phenolic compounds and flavonoids accumulated in E. trifolii-infected PR accessions (6–48 HAI). For rust, APX activity rose at 48 HAI in PR accessions. These findings suggest that partial resistance (PR) in grass pea is characterized by restricted pathogen invasion and a dynamic regulation of reactive oxygen species (ROS) scavenging enzymes, with responses varying across pathosystems. This highlights the importance of pathogen-specific selection strategies to minimize the risk of resistance breakdown and promote durable disease resistance in breeding programs.
- Integrating natural variation through GWAS – genetics of drought and flood tolerance in grass pea reveal independent yet interconnected mechanismsPublication . Sanches, Matilde; Vuylsteke, Marnik; Santos, Carmen; Mhamdi, Amna; Araújo, Susana de Sousa; Breusegem, Frank Van; Patto, Maria Carlota VazGrass pea (Lathyrus sativus L.) is a grain legume of increasing importance in the Mediterranean region due to its outstanding tolerance to abiotic stresses such as salinity, heat, drought, and flooding, outperforming many other legume species. Despite established natural phenotypic variation in response to water-related stresses, the genetic basis of this resilience remains poorly understood, hindering precision breeding for single and combined stress tolerance. A genome-wide association study was conducted here to investigate the genetic architecture of water stress responses in grass pea. Previously, phenotypic data, including gas exchange, chlorophyll a fluorescence, photosynthetic pigments, leaf water status, and biomass partitioning traits, were assessed under well-watered, mild drought, and partial submergence conditions across 194 representative grass pea accessions worldwide. The data were associated with 5,651 single nucleotide polymorphisms (SNPs) using linear mixed models under a restricted maximum likelihood framework, incorporating population structure and the newly assembled L0007 genome. A total of 130 unique SNPs associated with at least one trait-treatment combination or with trait variation between stress and control conditions, providing a valuable resource for precision breeding of multi-stress tolerance in grass pea. The loci associated with drought and waterlogging were largely non-overlapping, suggesting distinct genetic bases for the two stress tolerances. However, some common mechanisms, such as redox regulation and carbohydrate metabolism, emerged among the identified candidate genes, highlighting some interconnectedness of biological pathways involved in grass pea responses to water stress.