Browsing by Author "Oliveira, Maria Conceição"
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- Metabolomic profile and biological properties of sea lavender (Limonium algarvense Erben) plants cultivated with aquaculture wastewaters: implications for its use in herbal formulations and food additivesPublication . Rodrigues, Maria João; Castañeda-Loaiza, Viana; Monteiro, Ivo; Pinela, José; Barros, Lillian; Abreu, Rui M.V.; Oliveira, Maria Conceição; Reis, Catarina; Soares, Florbela; Pousão-Ferreira, Pedro; Pereira, Catarina G.; Custódio, LuísaWater extracts from sea lavender (Limonium algarvense Erben) plants cultivated in greenhouse conditions and irrigated with freshwater and saline aquaculture effluents were evaluated for metabolomics by liquid chromatography-tandem high-resolution mass spectrometry (LC-HRMS/MS), and functional properties by in vitro and ex vivo methods. In vitro antioxidant methods included radical scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid (ABTS), ferric-reducing antioxidant power (FRAP), and copper and iron chelating assets. Flowers’ extracts had the highest compounds’ diversity (flavonoids and its derivatives) and strongest in vitro antioxidant activity. These extracts were further tested for ex vivo antioxidant properties by oxidative haemolysis inhibition (OxHLIA), lipid peroxidation inhibition by thiobarbituric acid reactive substances (TBARS) formation, and anti-melanogenic, anti-tyrosinase, anti-inflammation, and cytotoxicity. Extract from plants irrigated with 300 mM NaCl was the most active towards TBARS (IC50 = 81 g/mL) and tyrosinase (IC50 = 873 g/mL). In OxHLIA, the activity was similar for fresh- and saltwater-irrigated plants (300 mM NaCl; IC50 = 136 and 140 g/mL, respectively). Samples had no anti-inflammatory and anti-melanogenic abilities and were not toxic. Our results suggest that sea lavender cultivated under saline conditions could provide a flavonoid-rich water extract with antioxidant and anti-tyrosinase properties with potential use as a food preservative or as a functional ingredient in herbal supplements.
- Phenolic compounds and bioactivity ot husk trom different rice cultivarsPublication . Serrano, Carmo; Oliveira, Maria Conceição; Castanho, Ana; Ferreira, Isabel C.F.R.; Brites, CarlaThere is a growing interest in food industry to valorize by-products from food production waste, and husk represents near 20% of raw material for rice millers. This study aimed to characterize the rice husk phenolic compounds extracted from different varieties ("Maçarico", "Ronaldo", "Ceres"COTARROZ) that may contribute to valorize these by-products. The in vitro antioxidant properties of the phenolic extracts were also investigated. The phenolic composition of extracts under study was identified and fully characterized by HPLC-DAD-ESI -HRMS/MS. The in vitro antioxidant activity of the extracts was assayed by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method, and the ferric reducing antioxidant power (FRAP). The LC-DAD-MS profile of the three rice husk extracts presented as main compounds tricin derivatives, including tricin-glycosides and tricinlignans. Ceres husk presented the highest tricin compounds, while Maçarico husk showed lower amount of these compounds. The antioxidant capacity evaluated by DPPH and FRAP assays follow the same trend as the phenolic profiles. The results show that Ceres husk have potential to be valorized in order to provide flavonoids compounds that can increase paddy rice shelf life. Future works will be conducted in order to evaluate also their antibacterial and cytotoxic properties against tumor and non-tumor cells.